| Development of a homologous equine relaxin radioimmunoassay. | |
| | |
MedLine Citation:
|
PMID: 3732158 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Equine relaxin (eRlx) immunoactivity has previously been measured in the mare during pregnancy using the porcine relaxin (pRlx) RIA (pRlx-RIA). This was not the optimal system for measurement of eRlx because the dose-response curve obtained with equine plasma was not parallel to the pRlx standard curve. A homologous eRlx-RIA has been developed and used to measure relaxin immunoactivity during pregnancy and parturition in the mare. Highly purified eRlx was used for the generation of antiserum in rabbits, preparation of tracer, and as assay standards. A double antibody eRlx RIA (eRlx-RIA) was developed which was highly specific and sensitive (0.023 ng relaxin/tube). The dose-response curve obtained with eRlx plasma was parallel to the equine standard curve while there was no parallelism noted between the pRlx and eRlx standard curves. This assay was utilized to measure eRlx in samples collected during pregnancy which were previously measured for relaxin content in the pRlx-RIA. It was found that the two assay systems gave almost identical patterns of secretion throughout pregnancy. The two assays differed in the amount of relaxin measured, with the eRlx-RIA measuring considerably higher amounts during gestation than the pRlx assay. In oxytocin-induced foalings, the differences became greater with the equine assay measuring as much as 10-fold greater concentrations. |
| | |
Authors:
|
D R Stewart |
Related Documents
:
|
3131158 - Luteal function following ovarian stimulation in rhesus monkeys for in vitro fertilizat... 14516718 - Effect of gilt seropositivity to lawsonia intracellularis (li) on their offspring's ser... 16452728 - Changes in vascular leakage and expression of angiopoietins in the corpus luteum during... 11030228 - Early diagnosis of pregnancy in sows by ultrasound evaluation of embryo development and... 16104678 - First-trimester risk assessment: twin gestations. 8903918 - Resolving polyhydramnios: clinical significance and subsequent neonatal outcome. |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
|
Title: Endocrinology Volume: 119 ISSN: 0013-7227 ISO Abbreviation: Endocrinology Publication Date: 1986 Sep |
Date Detail:
|
Created Date: 1986-09-19 Completed Date: 1986-09-19 Revised Date: 2007-11-14 |
Medline Journal Info:
|
Nlm Unique ID: 0375040 Medline TA: Endocrinology Country: UNITED STATES |
Other Details:
|
Languages: eng Pagination: 1100-4 Citation Subset: AIM; IM |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Animals Female Horses Labor, Induced / veterinary Oxytocin / pharmacology Pregnancy Radioimmunoassay / methods Relaxin / analysis* |
| Grant Support | |
ID/Acronym/Agency:
|
HD-06386/HD/NICHD NIH HHS |
| Chemical | |
Reg. No./Substance:
|
50-56-6/Oxytocin; 9002-69-1/Relaxin |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Purification and characterization of equine relaxin.
Next Document: Estrogen regulation of the biological activity of the avian oviduct progesterone receptor and its ab...