Document Detail


Development of a highly sensitive and robust Cor a 9 specific enzyme-linked immunosorbent assay for the detection of hazelnut traces.
MedLine Citation:
PMID:  22093353     Owner:  NLM     Status:  In-Data-Review    
Abstract/OtherAbstract:
Allergy to tree nuts represents an acute health problem. Sensitized people can be inadvertently exposed to hidden allergens resulting from cross-contamination of foods. For this reason, reliable and highly sensitive analytical methods are needed to be developed for control and labeling of food ingredients and products. In the present paper we have proposed a new allergen specific sandwich-ELISA for hazelnut operated in optical and electrochemical modes. The ELISA was based on chicken egg yolk antibodies raised against a major hazelnut allergen, Cor a 9. The developed ELISA has a limit of detection in phosphate buffer of 4ngmL(-1). No significant cross-reactivity with peanut, wheat or other food ingredients has been detected. Extracts of blank control cookies did not show any false positive response and the limit of detection in cookies was estimated to be 0.1μg of hazelnut protein per g of food (0.1ppm). The ELISA protocol was successfully adapted to operate in electrochemical mode and it was applied for the detection of hazelnut traces in cookies.
Authors:
Stanislav A Trashin; Tatiana Cucu; Bart Devreese; Annemie Adriaens; Bruno De Meulenaer
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Publication Detail:
Type:  Journal Article     Date:  2011-10-06
Journal Detail:
Title:  Analytica chimica acta     Volume:  708     ISSN:  1873-4324     ISO Abbreviation:  Anal. Chim. Acta     Publication Date:  2011 Dec 
Date Detail:
Created Date:  2011-11-18     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0370534     Medline TA:  Anal Chim Acta     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  116-22     Citation Subset:  IM    
Copyright Information:
Copyright © 2011 Elsevier B.V. All rights reserved.
Affiliation:
Department of Food Safety and Food Quality, Coupure links 653, 9000 Ghent, Ghent University, Belgium; Department of Biochemistry and Microbiology, K.L. Ledeganckstraat 35, 9000 Ghent, Ghent University, Belgium.
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