| Development of a highly enantioselective capacitive immunosensor for the detection of alpha-amino acids. | |
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MedLine Citation:
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PMID: 17073432 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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This work describes a highly enantioselective and sensitive immunosensor for the detection of chiral amino acids based on capacitive measurement. The sensor was prepared by first binding mercaptoacetic acid to the surface of a gold electrode, followed by modification with tyramine utilizing carbodiimide activation. The hapten 4-amino-D-phenylalanine was then covalently immobilized onto the electrode by diazotization. Stereoselective binding of an anti-D-amino acid antibody to the hapten-modified sensor surface resulted in capacitance changes that were detected with high sensitivity by a potentiostatic step method. Using capacitance measurement, detection limits of 5 pg of antibody/mL were attained. The exquisite stereoselectivity of the antibody was also utilized in a competitive setup to quantitatively determine the concentration of the analyte d-phenylalanine in nonracemic samples containing both enantiomers of this amino acid. Trace impurities of d-phenylalanine as low as 0.001% could be detected. |
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Authors:
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Song Zhang; Jingjing Ding; Ying Liu; Jilie Kong; Oliver Hofstetter |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Analytical chemistry Volume: 78 ISSN: 0003-2700 ISO Abbreviation: Anal. Chem. Publication Date: 2006 Nov |
Date Detail:
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Created Date: 2006-10-31 Completed Date: 2007-04-19 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0370536 Medline TA: Anal Chem Country: United States |
Other Details:
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Languages: eng Pagination: 7592-6 Citation Subset: IM |
Affiliation:
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Department of Chemistry, Fudan University, Shanghai 200433, People's Republic of China. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Amino Acids
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analysis* Biosensing Techniques* Haptens Phenylalanine / analogs & derivatives Stereoisomerism |
| Chemical | |
Reg. No./Substance:
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0/Amino Acids; 0/Haptens; 2410-24-4/4-aminophenylalanine; 63-91-2/Phenylalanine |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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