Document Detail

Development of hemagglutinin subtype-specific reference antisera by DNA vaccination of chickens.
MedLine Citation:
PMID:  14575109     Owner:  NLM     Status:  MEDLINE    
Previously, we have shown that intramuscular vaccination of chickens with the eukaryotic expression vector (EEV), expressing the influenza H5 hemagglutinin (H) protein, can stimulate a measurable and protective antibody response. Based on these results, we cloned other H genes from Eurasian H5, North American and Eurasian H7, and H15 influenza viruses into the EEV for use in vaccination of chickens to produce reference antibodies for diagnostic purposes, such as the hemagglutination inhibition (HI) test. Three-week-old specific pathogen free (SPF) chickens were vaccinated with 100 microg of EEV mixed with a cationic lipid by intramuscular injection. Then the birds were boostered twice at monthly intervals after the original vaccination. Measurable antibody titers were present for most birds after 1 month and generally increased after each boost. To examine the cross reactivity of the sera with other subtypes, HI test was conducted with antigens prepared from 15 subtypes of influenza virus. Subtype specificity of the antisera prepared by DNA vaccination were comparable or better than the antisera prepared by traditional method using whole virus vaccination. Preparation of reference antisera by DNA vaccination holds good promise because it is safe and allows for the production of H specific antibodies without producing antibodies specific to other influenza viral proteins.
C W Lee; D A Senne; D L Suarez
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Avian diseases     Volume:  47     ISSN:  0005-2086     ISO Abbreviation:  Avian Dis.     Publication Date:  2003  
Date Detail:
Created Date:  2003-10-24     Completed Date:  2003-12-10     Revised Date:  2005-11-22    
Medline Journal Info:
Nlm Unique ID:  0370617     Medline TA:  Avian Dis     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1051-6     Citation Subset:  IM    
Southeast Poultry Research Laboratory, 934 College Station Road, Athens, GA 30605, USA.
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MeSH Terms
Antibodies, Viral / blood
Cross Reactions
Hemagglutination Inhibition Tests
Hemagglutinin Glycoproteins, Influenza Virus / classification,  immunology*
Immune Sera
Influenza A virus / classification,  immunology*
Influenza Vaccines / administration & dosage*
Influenza in Birds / immunology*,  prevention & control
Injections, Intramuscular
Poultry Diseases / immunology,  prevention & control
Sensitivity and Specificity
Time Factors
Vaccines, DNA / administration & dosage*
Reg. No./Substance:
0/Antibodies, Viral; 0/Hemagglutinin Glycoproteins, Influenza Virus; 0/Immune Sera; 0/Influenza Vaccines; 0/Vaccines, DNA

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