Document Detail


Development of glycinergic synaptic transmission to rat brain stem motoneurons.
MedLine Citation:
PMID:  9819267     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Using an in vitro rat brain stem slice preparation, we examined the postnatal changes in glycinergic inhibitory postsynaptic currents (IPSCs) and passive membrane properties that underlie a developmental change in inhibitory postsynaptic potentials (IPSPs) recorded in hypoglossal motoneurons (HMs). Motoneurons were placed in three age groups: neonate (P0-3), intermediate (P5-8), and juvenile (P10-18). During the first two postnatal weeks, the decay time course of both unitary evoked IPSCs [mean decay time constant, taudecay = 17.0 +/- 1.6 (SE) ms in neonates and 5.5 +/- 0.4 ms in juveniles] and spontaneous miniature IPSCs (taudecay = 14.2 +/- 2.4 ms in neonates and 6.3 +/- 0.7 ms in juveniles) became faster. As glycine uptake does not influence IPSC time course at any postnatal age, this change most likely results from a developmental alteration in glycine receptor (GlyR) subunit composition. We found that expression of fetal (alpha2) GlyR subunit mRNA decreased, whereas expression of adult (alpha1) GlyR subunit mRNA increased postnatally. Single GlyR-channels recorded in outside-out patches excised from neonate motoneurons had longer mean burst durations than those from juveniles (18.3 vs. 11.1 ms). Concurrently, HM input resistance (RN) and membrane time constant (taum) decreased (RN from 153 +/- 12 MOmega to 63 +/- 7 MOmega and taum from 21.5 +/- 2.7 ms to 9.1 +/- 1.0 ms, neonates and juveniles, respectively), and the time course of unitary evoked IPSPs also became faster (taudecay = 22.4 +/- 1.8 and 7.7 +/- 0.9 ms, neonates vs. juveniles, respectively). Simulated synaptic currents were used to probe more closely the interaction between IPSC time course and taum, and these simulations demonstrated that IPSP duration was reduced as a consequence of postnatal changes in both the kinetics of the underlying GlyR channel and the membrane properties that transform the IPSC into a postsynaptic potential. Additionally, gramicidin perforated-patch recordings of glycine-evoked currents reveal a postnatal change in reversal potential, which is shifted from -37 to -73 mV during this same period. Glycinergic PSPs are therefore depolarizing and prolonged in neonate HMs and become faster and hyperpolarizing during the first two postnatal weeks.
Authors:
J H Singer; E M Talley; D A Bayliss; A J Berger
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Publication Detail:
Type:  In Vitro; Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Journal of neurophysiology     Volume:  80     ISSN:  0022-3077     ISO Abbreviation:  J. Neurophysiol.     Publication Date:  1998 Nov 
Date Detail:
Created Date:  1998-12-08     Completed Date:  1998-12-08     Revised Date:  2013-01-29    
Medline Journal Info:
Nlm Unique ID:  0375404     Medline TA:  J Neurophysiol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  2608-20     Citation Subset:  IM    
Affiliation:
Department of Physiology and Biophysics, University of Washington School of Medicine, Seattle, Washington 98195-7290, USA.
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MeSH Terms
Descriptor/Qualifier:
Animals
Animals, Newborn
Brain Stem / cytology,  growth & development,  physiology*
Cell Membrane / physiology
Excitatory Postsynaptic Potentials / physiology
Female
Glycine / pharmacology
In Situ Hybridization
Kinetics
Male
Motor Neurons / physiology*
Neural Inhibition / physiology
Patch-Clamp Techniques
RNA, Messenger / biosynthesis
Rats
Rats, Sprague-Dawley
Receptors, Glycine / biosynthesis,  genetics,  physiology*
Synaptic Transmission / physiology*
Grant Support
ID/Acronym/Agency:
HL-49657/HL/NHLBI NIH HHS; NS-14857/NS/NINDS NIH HHS; NS-33583/NS/NINDS NIH HHS; R01 NS033583/NS/NINDS NIH HHS
Chemical
Reg. No./Substance:
0/RNA, Messenger; 0/Receptors, Glycine; 56-40-6/Glycine

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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