Document Detail


Development of a fully integrated microfluidic system for sensing infectious viral disease.
MedLine Citation:
PMID:  18551711     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
An active micromixer system utilizing the magnetic force was developed and examined for its ability to facilitate the mixing of more than two fluid flows. The mixing performance of the active micromixer was evaluated in aqueous-aqueous systems including dyes for visual observation. A complete analytical microfluidic system was developed by integrating various functional modules into a single chip, thus allowing cell lysis, sample preparation, purification of intracellular molecules, and subsequent analysis. Upon loading the cell samples and lysis solution into the mixing chamber, the integrated microfluidic device allows efficient cell disruption by rotation of a micromagnetic disk and control of mixing time using the Teflon-coated hydrophobic film as a microvalve. This inflow is followed by separating the cell debris and contaminated proteins from the cell lysate sample using the acrylamide (AAm)-functionalized SPE. The inflow of partially purified cell lysate sample containing the gold binding polypeptide (GBP)-fusion protein was bound onto the gold micropatterns by means of its metal binding affinity. The GBP-fusion method allows immobilization of proteins in bioactive forms onto the gold surface without surface modification suitable for studying antigen-antibody interaction. It was used for the detection of severe acute respiratory syndrome (SARS), an infectious viral disease, as an example case.
Authors:
Yun Suk Huh; Tae Jung Park; Eun Zoo Lee; Won Hi Hong; Sang Yup Lee
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Electrophoresis     Volume:  29     ISSN:  0173-0835     ISO Abbreviation:  Electrophoresis     Publication Date:  2008 Jul 
Date Detail:
Created Date:  2008-07-29     Completed Date:  2008-09-30     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8204476     Medline TA:  Electrophoresis     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  2960-9     Citation Subset:  IM    
Affiliation:
Department of Chemical and Biomolecular Engineering (BK21 program), KAIST, Yuseong-gu, Daejeon, Korea.
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MeSH Terms
Descriptor/Qualifier:
Electrochemistry
Escherichia coli / genetics
Green Fluorescent Proteins / genetics
Humans
Microfluidics / instrumentation*
Microscopy, Atomic Force
Recombinant Fusion Proteins / genetics
Virus Diseases / diagnosis*
Chemical
Reg. No./Substance:
0/Recombinant Fusion Proteins; 147336-22-9/Green Fluorescent Proteins

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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