| Development of fructosyl valine binding polymers by covalent imprinting. | |
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MedLine Citation:
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PMID: 17419046 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Molecularly imprinted polymers (MIPs) against fructosyl valine (Fru-Val), the N-terminal constituent of hemoglobin A1c beta-chains, were prepared by cross-linking of beta-D-Fru-Val-O-bis(4-vinylphenylboronate) with an excess of ethylene glycol dimethacrylate (EDMA) or trimethylolpropane trimethacrylate (TRIM). Control MIPs were prepared in analogy by cross-linking the corresponding vinylphenylboronate esters of fructose and pinacol. After template extraction batch rebinding studies were performed using different pH values and buffer compositions. The Fru-Val imprinted TRIM cross-linked polymer binds about 1.4 times more Fru-Val than the fructose imprinted polymer and 2.7 times more Fru-Val than pinacol imprinted polymer. The highest imprinting effect was obtained in 100 mM sodium carbonate/10% methanol (pH 11.4). The TRIM cross-linked Fru-Val imprinted polymer showed a better specificity than the EDMA cross-linked polymer. The binding of valine was very low. Thermo gravimetric analysis indicated that the generated Fru-Val imprinted polymer has high thermo stability. No change in binding was observed after incubation of the polymers in buffer at 80 degrees C for 36 h. Since the functional group of the polymers (phenyl boronic acid) targets the sugar part of Fru-Val the imprint technique used should also be applicable for the development of MIPs against other glycated amino acids and peptides. |
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Authors:
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Rajagopal Rajkumar; Axel Warsinke; Helmuth Möhwald; Frieder W Scheller; Martin Katterle |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2007-03-07 |
Journal Detail:
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Title: Biosensors & bioelectronics Volume: 22 ISSN: 0956-5663 ISO Abbreviation: Biosens Bioelectron Publication Date: 2007 Jun |
Date Detail:
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Created Date: 2007-05-21 Completed Date: 2007-07-26 Revised Date: 2009-07-14 |
Medline Journal Info:
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Nlm Unique ID: 9001289 Medline TA: Biosens Bioelectron Country: England |
Other Details:
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Languages: eng Pagination: 3318-25 Citation Subset: IM |
Affiliation:
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Analytical Biochemistry, Potsdam University, D-14476 Potsdam, Germany. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Hemoglobin A, Glycosylated
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analysis* Humans Hydroxylamines Methacrylates / chemistry* Polymers / chemistry* Temperature Valine / analogs & derivatives*, chemistry |
| Chemical | |
Reg. No./Substance:
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0/Hemoglobin A, Glycosylated; 0/Hydroxylamines; 0/Methacrylates; 0/N,O-dimethacryloylhydroxylamine; 0/Polymers; 0/hemoglobin A1c protein, human; 10003-64-2/fructosylvaline; 3290-92-4/trimethylolpropane trimethacrylate; 7004-03-7/Valine; 97-90-5/ethylene dimethacrylate |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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