Document Detail

Development of a fluorescent microsphere technique for rapid histological determination of cerebral blood flow.
MedLine Citation:
PMID:  20193669     Owner:  NLM     Status:  MEDLINE    
The purpose of this study was to develop a more efficient fluorescent microsphere method to facilitate the rapid use of the histological technique and to enable its use in large tissue regions. Using fluorescent plate/slide imaging technology and automated detection and analysis software, we were able to rapidly image, detect, and count 3 separate microsphere colors in 200 microm thick tissue sections from piglet brain. In resting newborn piglets (n=6) on isoflurane anesthesia, we measured a median total cerebral blood flow (CBF) of 105 ml/min/100g (range 27-206 ml/min/100 g). Compared with other FM analysis methods, our method reduces the time required to determine blood flow, improves accuracy in lipid-rich tissues and large tissue regions and, unlike the radiolabeled microsphere method, can be combined with histological analysis.
Stephanie A Eucker; Brenton D Hoffman; Rahul Natesh; Jill Ralston; William M Armstead; Susan S Margulies
Related Documents :
24595019 - Chong meridian 衝脈 an ancient chinese description of the vascular system?
23436399 - Objective qualitative and quantitative assessment of blood flow with near-infrared angi...
3916789 - Regulation of the peripheral vasculature and tissue oxygenation in health and disease.
20843539 - Dynamic changes of α-hexachlorocyclohexane and its enantiomers in various tissues of ja...
17015239 - Contrast-enhanced ultrasound for examining tumor biology.
25064929 - E-014 changes in posterior circulation arterial flows after revascularization surgery i...
971549 - Effects of quinidine on blood flow rate and developed tension in blood-perfused canine ...
170829 - Effects of estrogens on systemic and regional circulations in normal and renal hyperten...
9818439 - A perception of transfusion safety and risks.
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't     Date:  2010-03-01
Journal Detail:
Title:  Brain research     Volume:  1326     ISSN:  1872-6240     ISO Abbreviation:  Brain Res.     Publication Date:  2010 Apr 
Date Detail:
Created Date:  2010-04-05     Completed Date:  2010-06-25     Revised Date:  2014-09-20    
Medline Journal Info:
Nlm Unique ID:  0045503     Medline TA:  Brain Res     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  128-34     Citation Subset:  IM    
Copyright Information:
Copyright 2010 Elsevier B.V. All rights reserved.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Blood Pressure / physiology
Brain / blood supply*
Cerebrovascular Circulation / physiology*
Image Processing, Computer-Assisted / methods
Microscopy, Fluorescence / methods*
Regional Blood Flow / physiology
Grant Support

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Frequency-dependent changes in synaptic plasticity and brain-derived neurotrophic factor (BDNF) expr...
Next Document:  A new genetic model of activity-induced Ras signaling dependent pre-synaptic plasticity in Drosophil...