Document Detail


Development of a fluorescent microsphere technique for rapid histological determination of cerebral blood flow.
MedLine Citation:
PMID:  20193669     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The purpose of this study was to develop a more efficient fluorescent microsphere method to facilitate the rapid use of the histological technique and to enable its use in large tissue regions. Using fluorescent plate/slide imaging technology and automated detection and analysis software, we were able to rapidly image, detect, and count 3 separate microsphere colors in 200 microm thick tissue sections from piglet brain. In resting newborn piglets (n=6) on isoflurane anesthesia, we measured a median total cerebral blood flow (CBF) of 105 ml/min/100g (range 27-206 ml/min/100 g). Compared with other FM analysis methods, our method reduces the time required to determine blood flow, improves accuracy in lipid-rich tissues and large tissue regions and, unlike the radiolabeled microsphere method, can be combined with histological analysis.
Authors:
Stephanie A Eucker; Brenton D Hoffman; Rahul Natesh; Jill Ralston; William M Armstead; Susan S Margulies
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't     Date:  2010-03-01
Journal Detail:
Title:  Brain research     Volume:  1326     ISSN:  1872-6240     ISO Abbreviation:  Brain Res.     Publication Date:  2010 Apr 
Date Detail:
Created Date:  2010-04-05     Completed Date:  2010-06-25     Revised Date:  2014-09-20    
Medline Journal Info:
Nlm Unique ID:  0045503     Medline TA:  Brain Res     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  128-34     Citation Subset:  IM    
Copyright Information:
Copyright 2010 Elsevier B.V. All rights reserved.
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MeSH Terms
Descriptor/Qualifier:
Animals
Blood Pressure / physiology
Brain / blood supply*
Cerebrovascular Circulation / physiology*
Female
Image Processing, Computer-Assisted / methods
Microscopy, Fluorescence / methods*
Microspheres*
Regional Blood Flow / physiology
Swine
Grant Support
ID/Acronym/Agency:
R01 NS039679/NS/NINDS NIH HHS; R01 NS039679/NS/NINDS NIH HHS; R01 NS039679-08/NS/NINDS NIH HHS
Comments/Corrections

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