| Development of a fluorescent method for detecting the onset of coagulation in human plasma on microstructured lateral flow platforms. | |
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MedLine Citation:
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PMID: 21293802 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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Microfluidic devices and microsystems have been used to develop blood coagulation monitoring devices for point of care diagnostic use. However, many of them suffer from inherent variability and imprecision, partly due to the fact that they only detect changes in bulk clotting properties and do not reflect the microscopic nature of blood coagulation. This work demonstrates microstructured lateral flow platforms used in combination with fluorescently labelled fibrinogen to detect microscopic clot formation. Plasma samples applied to platforms modified with coagulation activation reagents and fluorescent fibrinogen produced changes in fluorescence intensity due to incorporation of the fluorophore into the forming microclots. It was found that the change in the distribution of the fluorescence within the sample over time was an excellent predictor of the onset of coagulation, which could be used to determine the clotting time. The impact of various assay parameters was optimised and the assay was shown to be capable of measuring the effect of heparin concentration on blood clotting time from 0 to 1.5 U mL(-1). |
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Authors:
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Magdalena M Dudek; Nigel J Kent; Pan Gu; Z Hugh Fan; Anthony J Killard |
Publication Detail:
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Type: JOURNAL ARTICLE Date: 2011-2-4 |
Journal Detail:
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Title: The Analyst Volume: - ISSN: 1364-5528 ISO Abbreviation: - Publication Date: 2011 Feb |
Date Detail:
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Created Date: 2011-2-4 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0372652 Medline TA: Analyst Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Affiliation:
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Biomedical Diagnostics Institute, National Centre for Sensor Research, Dublin City University, Dublin, 9, Ireland. tony.killard@uwe.ac.uk. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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