Document Detail


Development of fluorescence imaging-based assay for screening cardioprotective compounds from medicinal plants.
MedLine Citation:
PMID:  21819864     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Medicinal plants have been widely recognized as a renewable resource for the discovery of novel leads and drug. In this study, an approach for screening and identification compounds with cardioprotective activity from medicinal plant extracts by cellular-fluorescence imaging technique was developed. It is a cell-based assay for measuring mitochondrial membrane potential changes in H9c2 cardiac muscle cells exposed to H(2)O(2) by using a fluorescence automatic microscopy screening platform. Rhodamine 123 was used as the fluorescent dye to indicate the change of mitochondrial membrane potential. The sensitivity and linear range of the proposed approach were evaluated and validated using vitamin C, an antioxidative compound. The method was applied to screen active components with potent cardioprotective effects from a traditional Chinese formula. The potential cardioprotective components were identified by liquid chromatography coupled with mass spectrometry (LC/MS). Moreover, the utility of the proposed approach was further validated by three compounds (salvianolic acid B, protocatechuic aldehyde, and tanshinone II A) identified from the formula which showed cardioprotective effects in a dose-dependent manner. These applications suggested that the proposed rapid and sensitive screening approach offers an efficient way to discover active components or compounds from medicinal plants.
Authors:
Yi Wang; Xiaoping Zhao; Xiumei Gao; Xiaojing Nie; Yingxin Yang; Xiaohui Fan
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2011-06-23
Journal Detail:
Title:  Analytica chimica acta     Volume:  702     ISSN:  1873-4324     ISO Abbreviation:  Anal. Chim. Acta     Publication Date:  2011 Sep 
Date Detail:
Created Date:  2011-08-08     Completed Date:  2011-11-23     Revised Date:  2012-01-09    
Medline Journal Info:
Nlm Unique ID:  0370534     Medline TA:  Anal Chim Acta     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  87-94     Citation Subset:  IM    
Copyright Information:
Copyright © 2011 Elsevier B.V. All rights reserved.
Affiliation:
Pharmaceutical Informatics Institute, Zhejiang University, Hangzhou 310058, PR China.
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MeSH Terms
Descriptor/Qualifier:
Animals
Ascorbic Acid / pharmacology
Cardiotonic Agents / chemistry*,  isolation & purification*
Cell Line / cytology
Chromatography, Liquid / methods
Drug Discovery / methods*
Drug Evaluation, Preclinical / methods
Drugs, Chinese Herbal / pharmacology,  therapeutic use
Fluorescence
High-Throughput Screening Assays / methods*
Hydrogen Peroxide / pharmacology*
Mass Spectrometry / methods
Medicine, Chinese Traditional
Membrane Potential, Mitochondrial / drug effects*
Microscopy, Fluorescence / methods
Plants, Medicinal / chemistry*
Rats
Rhodamine 123 / analysis
Chemical
Reg. No./Substance:
0/Cardiotonic Agents; 0/Drugs, Chinese Herbal; 0/qishen yiqi; 50-81-7/Ascorbic Acid; 62669-70-9/Rhodamine 123; 7722-84-1/Hydrogen Peroxide
Comments/Corrections
Erratum In:
Anal Chim Acta. 2012 Jan 13;710:131

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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