| Development of a fibrin composite-coated poly(epsilon-caprolactone) scaffold for potential vascular tissue engineering applications. | |
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MedLine Citation:
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PMID: 18546199 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Poor cell adhesion, cytotoxicity of degradation products and lack of biological signals for cell growth, survival, and tissue generation are the limitations in the use of a biodegradable polymer scaffold for vascular tissue engineering. We have fabricated a hybrid scaffold by integrating physicochemical characteristics of poly(epsilon-caprolactone) (PCL) and biomimetic property of a composite of fibrin, fibronectin, gelatin, growth factors, and proteoglycans to improve EC growth on the scaffold. Solvent cast porous films of poly(epsilon-caprolactone) was prepared using PEG as a porogen. Porosity varied between 5 and 200 microm, and FTIR spectroscopy confirmed structural aspects of PCL. Films kept in PBS for 60 days showed tensile strength and elongation matching native blood vessel. Slow degradation of the scaffold was demonstrated by gravimetric analysis and molecular weight determination. Human umbilical vein endothelial cell (HUVEC) adhesion and proliferation on bare films were minimal. FTIR spectroscopy and environmental scanning electron microscopy (ESEM) of PCL-fibrin hybrid scaffold confirmed the presence of fibrin composite on PCL film. HUVEC was subsequently cultured on hybrid scaffold, and continuous EC lining was observed in 15 and 30 days of culture using ESEM. Results suggest that the new hybrid scaffold can be a suitable candidate for cardiovascular tissue engineering. |
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Authors:
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Divya Pankajakshan; Lizymol P Philipose; Minshiya Palakkal; Kalliyana Krishnan; Lissy K Krishnan |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Journal of biomedical materials research. Part B, Applied biomaterials Volume: 87 ISSN: 1552-4981 ISO Abbreviation: J. Biomed. Mater. Res. Part B Appl. Biomater. Publication Date: 2008 Nov |
Date Detail:
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Created Date: 2008-10-13 Completed Date: 2009-01-30 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 101234238 Medline TA: J Biomed Mater Res B Appl Biomater Country: United States |
Other Details:
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Languages: eng Pagination: 570-9 Citation Subset: IM |
Affiliation:
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Thrombosis Research Unit, Biomedical Technology Wing, Sree Chitra Tirunal Institute for Medical Sciences and Technology, Poojappura, Trivandrum 695 012, India. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Cells, Cultured Endothelial Cells Fibrin / chemistry* Humans Molecular Weight Polyesters / chemistry* Porosity Spectroscopy, Fourier Transform Infrared Stress, Mechanical Tissue Engineering / methods* Viscosity |
| Chemical | |
Reg. No./Substance:
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0/Polyesters; 24980-41-4/polycaprolactone; 9001-31-4/Fibrin |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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