| Development and evaluation of flow through technique for diagnosis of cystic echinococcosis in sheep. | |
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MedLine Citation:
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PMID: 21507576 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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A novel in vitro flow through technique was developed and evaluated for immunodiagnosis of cystic echinococcosis in sheep using hydatid specific non-cross reactive 8-kDa protein. The 8-kDa protein was prepared from hydatid cyst fluid by DEAE-Sepharose fast flow anion exchange chromatography. In this flow through technique, the 8-kDa antigen was coated on the nitrocellulose membrane of flow through device. Protein A colloidal gold was used as detector. The evaluation of the technique was performed by comparing 150 known positive hydatid serum and known negative serum collected from sheep. The test was shown to be high sensitivity and specificity that were closely correlated with those of EITB. Furthermore the immunofiltration-based assay is rapid (2min) and easy to perform with no requirement of special skill, reagent and instrumentation. This suggests the flow through technique is an acceptance alternative to be used in clinical laboratories lacking specialized equipments as well as large scale screening of cystic echinococcosis both in the field with animal and human populations. |
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Authors:
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N Jeyathilakan; S Abdul Basith; Lalitha John; N Daniel Joy Chandran; G Dhinakar Raj |
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Publication Detail:
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Type: JOURNAL ARTICLE Date: 2011-3-31 |
Journal Detail:
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Title: Veterinary parasitology Volume: - ISSN: 1873-2550 ISO Abbreviation: - Publication Date: 2011 Mar |
Date Detail:
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Created Date: 2011-4-21 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 7602745 Medline TA: Vet Parasitol Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Copyright Information:
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Copyright © 2011 Elsevier B.V. All rights reserved. |
Affiliation:
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Department of Veterinary Parasitology, Madras Veterinary College, Vepery, Chennai 600007, Tamil Nadu, India. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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