Document Detail


Development and evaluation of an enzyme linked immunotransfer blot technique for serodiagnosis of Chagas' disease.
MedLine Citation:
PMID:  7716393     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
An enzyme linked immunoelectrotransfer blot technique (EITB) was developed and evaluated for the serodiagnosis of Chagas' disease. EITB strips were prepared using Trypansoma cruzi (Y strain) epimastigotes lysate. Evaluation was performed with 235 serum samples collected from individuals living in an endemic area for Chagas' disease. Among those samples, 160 were serological positive in three conventional tests for T. cruzi and 75 were negative. The specificity was determined using 37 serum samples from patients with other infectious diseases. The EITB test showed sensitivity of 99.3% and specificity of 100% when a positive reaction was defined as the presence of 3 bands from a group of 7 (14, 19, 27, 30, 34, 37 and 75), a negative reaction was defined as the absence of 6 of the 7 bands, and an indeterminate reaction as the presence of two of the 7 bands.
Authors:
M G Teixeira; J Borges-Pereira; E Netizert; M L Souza; J M Peralta
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Tropical medicine and parasitology : official organ of Deutsche Tropenmedizinische Gesellschaft and of Deutsche Gesellschaft für Technische Zusammenarbeit (GTZ)     Volume:  45     ISSN:  0177-2392     ISO Abbreviation:  Trop. Med. Parasitol.     Publication Date:  1994 Dec 
Date Detail:
Created Date:  1995-05-17     Completed Date:  1995-05-17     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8503728     Medline TA:  Trop Med Parasitol     Country:  GERMANY    
Other Details:
Languages:  eng     Pagination:  308-12     Citation Subset:  IM    
Affiliation:
Instituto de Microbiologia, UFRJ, Rio de Janeiro, São Paulo, Brazil.
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MeSH Terms
Descriptor/Qualifier:
Chagas Disease / diagnosis*
Humans
Immunoblotting
Serologic Tests

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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