Document Detail


Development of an enzyme-modified carbon paste electrode for determining inhibitors of lipoxygenase.
MedLine Citation:
PMID:  16202879     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
In this study, a 15-lipoxygenase-modified carbon paste electrode (15-LOX-MCPE) was developed in connection with the help of voltammetry, which can be used as an assay system for screening drugs with inhibiting lipoxygenase (LOX) activity. The influence of different experimental conditions (LOX loading of carbon paste, pH, type of buffer system etc.) was investigated in order to optimise the biosensing device. The best composition of the biosensor is 30% paraffin oil, 68% graphite powder and 2% LOX. The optimised voltammetric measurement medium is Sörensen/NaOH (0.1M, pH 9.0) using linoleic acid as a substrate. Under these conditions the hydroperoxy linoleic acid is formed, which can be oxidised at a potential of +0.9 V versus Ag/AgCl/3M KCl. The applicability of the LOX biosensor as assay of lipoxygenase inhibitors was successfully tested with nordihydroguaiaretic acid, zileuton and fenleuton, which are well known inhibitors of LOX.
Authors:
Anna Rittmannsberger; Werner Likussar; Astrid Michelitsch
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Publication Detail:
Type:  Evaluation Studies; Journal Article     Date:  2005-01-08
Journal Detail:
Title:  Biosensors & bioelectronics     Volume:  21     ISSN:  0956-5663     ISO Abbreviation:  Biosens Bioelectron     Publication Date:  2005 Oct 
Date Detail:
Created Date:  2005-10-05     Completed Date:  2006-02-15     Revised Date:  2009-07-14    
Medline Journal Info:
Nlm Unique ID:  9001289     Medline TA:  Biosens Bioelectron     Country:  England    
Other Details:
Languages:  eng     Pagination:  655-60     Citation Subset:  IM    
Affiliation:
Institute of Pharmaceutical Sciences, Karl-Franzens-University Graz, Schubertstrasse 1, A-8010 Graz, Austria.
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MeSH Terms
Descriptor/Qualifier:
Arachidonate 15-Lipoxygenase / analysis,  antagonists & inhibitors*
Biosensing Techniques / instrumentation*,  methods
Carbon / chemistry*
Electrochemistry / instrumentation*,  methods
Electrodes*
Enzymes, Immobilized / chemistry
Equipment Design
Equipment Failure Analysis
Lipoxygenase Inhibitors / analysis*,  chemistry
Ointments
Chemical
Reg. No./Substance:
0/Enzymes, Immobilized; 0/Lipoxygenase Inhibitors; 0/Ointments; 7440-44-0/Carbon; EC 1.13.11.33/Arachidonate 15-Lipoxygenase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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