| Development of enzyme-linked immunosorbent assays based on recombinant MSP1a and MSP2 of Anaplasma marginale. | |
| | |
MedLine Citation:
|
PMID: 16410967 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Indirect enzyme-linked immunosorbent assays (ELISAs) based on recombinant MSP1a and MSP2 from a Brazilian isolate of Anaplasma marginale were developed to detect antibodies against this rickettsia in cattle. The high sensitivities (99% for both tests) and specificities (100% for both tests) were confirmed with sera from cattle positive or negative for A. marginale antibodies, respectively, by immunofluorescent antibody test. By the analysis of 583 sera from cattle of three regions of the state of Pernambuco, Brazil, the agreement between both tests was high, with a kappa index of 0.89. The similar performances of the ELISAs suggest that both tests can be used in epidemiological surveys for detection of antibodies to A. marginale in cattle. |
| | |
Authors:
|
Flábio R Araújo; Valeska S P Melo; Carlos A N Ramos; Claudio R Madruga; Cleber O Soares; Raul H Kessler; Nalvo F Almeida; Graziela S Araújo; Leucio C Alves; Roberto A A Torres Júnior; Stênio P Fragoso; Paulo R C Arauco; Gisele Bacanelli; Maristeli B Oliveira; Lenita R Santos |
Related Documents
:
|
10383077 - A comparison of elisa assays as routine diagnostic test for detection of autoantibodies... 17133547 - Rapid immunochromatographic test for hantavirus andes contrasted with capture-igm elisa... 10882907 - Prevalence of antibodies to toxoplasma gondii in the sera of rare wildlife in the shang... 2555377 - An improved elisa and serum neutralisation test for the detection of turkey rhinotrache... 15543417 - Study of the antibody response against mycobacterium tuberculosis antigens in warao ame... 18796897 - The effect of inter-trial rest interval on the assessment of isokinetic thigh muscle to... |
Publication Detail:
|
Type: Comparative Study; Evaluation Studies; Journal Article; Research Support, Non-U.S. Gov't Date: 2006-01-09 |
Journal Detail:
|
Title: Memórias do Instituto Oswaldo Cruz Volume: 100 ISSN: 0074-0276 ISO Abbreviation: Mem. Inst. Oswaldo Cruz Publication Date: 2005 Nov |
Date Detail:
|
Created Date: 2006-01-13 Completed Date: 2006-02-21 Revised Date: 2006-11-15 |
Medline Journal Info:
|
Nlm Unique ID: 7502619 Medline TA: Mem Inst Oswaldo Cruz Country: Brazil |
Other Details:
|
Languages: eng Pagination: 765-9 Citation Subset: IM |
Affiliation:
|
Embrapa Gado de Corte, Rodovia BR 262, 79002-970 Campo Grande, MS, Brazil. flabio@cnpgc.embrapa.br |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Anaplasma marginale
/
immunology* Anaplasmosis / diagnosis* Animals Antibodies, Bacterial / blood* Antigens, Bacterial / genetics, immunology* Bacterial Outer Membrane Proteins / genetics, immunology* Cattle Cattle Diseases / diagnosis*, microbiology Enzyme-Linked Immunosorbent Assay / veterinary* Fluorescent Antibody Technique Recombinant Proteins / genetics, immunology Sensitivity and Specificity |
| Chemical | |
Reg. No./Substance:
|
0/Antibodies, Bacterial; 0/Antigens, Bacterial; 0/Bacterial Outer Membrane Proteins; 0/Recombinant Proteins; 0/major surface protein 1a, Anaplasma; 0/msp2 protein, Anaplasma marginale |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: A method for testing the host specificity of ectoparasites: give them the opportunity to choose.
Next Document: Efficacy of Citrus reticulata and Mirazid in treatment of Schistosoma mansoni.