Document Detail

Development of enzyme-linked immunosorbent assays for isocupressic acid and serum metabolites of isocupressic acid.
MedLine Citation:
PMID:  12744647     Owner:  NLM     Status:  MEDLINE    
The consumption of ponderosa pine (Pinus ponderosa), lodgepole pine (Pinus contorta), common juniper (Juniperus communis), and Monterey cypress (Cupressus macrocarpa) causes abortions in pregnant cattle. Recent studies have identified isocupressic acid (1) as the primary abortificient compound in these plants. In vitro and in vivo studies using rumen and blood have shown isocupressic acid (1) is rapidly metabolized to agathic acid (3), dihydroagathic acid (4), and tetrahydroagathic acid (5). Rapid and sensitive diagnostic techniques are needed to identify poisoned animals, to study toxicokinetics, and to elucidate the mechanism of isocupressic acid-induced abortion in cattle. In this study, four competitive inhibition enzyme-linked immunosorbent assays for isocupressic acid and its sera metabolites were developed using polyclonal antibodies. One assay is specific to 1, whereas the other three assays show cross-reactivity to 3-5 in addition to 1. The assay specific to 1 had a limit of detection of 44.1 pg. The other assays which demonstrated cross-reactivity to the isocupressic acid blood metabolites also had comparably low limits of detection. One assay was used to follow the absorption and elimination profile of isocupressic acid metabolites in both cow serum and urine after oral dosage of a cow with common juniper.
Stephen T Lee; Dale R Gardner; Massoud Garrosian; Kip E Panter; Alessio N Serrequi; Thomas K Schoch; Bryan L Stegelmeier
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Journal of agricultural and food chemistry     Volume:  51     ISSN:  0021-8561     ISO Abbreviation:  J. Agric. Food Chem.     Publication Date:  2003 May 
Date Detail:
Created Date:  2003-05-14     Completed Date:  2003-07-17     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  0374755     Medline TA:  J Agric Food Chem     Country:  United States    
Other Details:
Languages:  eng     Pagination:  3228-33     Citation Subset:  IM    
Poisonous Plant Research Laboratory, Agricultural Research Service, U.S. Department of Agriculture, 1150 E. 1400 N., Logan, Utah 84341, USA.
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MeSH Terms
Abortifacient Agents / analysis,  blood
Carboxylic Acids / analysis*,  blood*,  urine
Diterpenes / analysis*,  blood*,  urine
Enzyme-Linked Immunosorbent Assay / methods*
Juniperus / chemistry
Pinus / chemistry
Plant Bark / chemistry
Sensitivity and Specificity
Tetrahydronaphthalenes / analysis*,  blood*,  urine
Grant Support
Reg. No./Substance:
0/Abortifacient Agents; 0/Carboxylic Acids; 0/Diterpenes; 0/Tetrahydronaphthalenes; 1909-91-7/isocupressic acid

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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