Document Detail


Development of enzyme-linked immunosorbent assay for the detection of bt protein in transgenic cotton.
MedLine Citation:
PMID:  23143488     Owner:  NLM     Status:  In-Data-Review    
Abstract/OtherAbstract:
Enzyme-linked immunosorbent assays (ELISA) are being used extensively for the identification of Bt-protein in Bt transgenic crops. A sandwich ELISA test is the most preferable immunoassay for the quantification of Bt-protein in transgenic cotton plants. Here, we describe development of sandwich ELISA, employing polyclonal rabbit antibody as a capture antibody and HRP-labeled mouse anti-Bt protein-antibody as a detector antibody.
Authors:
Suchitra Kamle; Abhishek Ojha; Arvind Kumar
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Methods in molecular biology (Clifton, N.J.)     Volume:  958     ISSN:  1940-6029     ISO Abbreviation:  Methods Mol. Biol.     Publication Date:  2013  
Date Detail:
Created Date:  2012-11-12     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9214969     Medline TA:  Methods Mol Biol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  131-8     Citation Subset:  IM    
Affiliation:
Molecular Genomics Laboratory, National Institute of Immunology, New Delhi, India, suchitrakamle@gmail.com.
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