Document Detail


Development of an enzyme-linked immunosorbent assay for immunoglobulin M antibodies against measles virus.
MedLine Citation:
PMID:  12738645     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Measles is a highly contagious respiratory virus infection, with typical clinical symptoms including maculopapular rash, fever, cough, coryza, and conjunctivitis. Despite implementation of widespread vaccination programs throughout the world, the rates of global morbidity and mortality are still considerable. This study was performed to design a reliable indirect enzyme-linked immunosorbent assay (ELISA) to measure measles-specific immunoglobulin M (IgM). First, human IgM was purified, and then an anti-IgM antibody was produced in rabbits and purified in a multistep process. The rabbit IgG against human IgM was conjugated with peroxidase. Measles virus-infected Vero cells produced viral antigen. One hundred serum samples from infants of 9 to 18 months of age, mostly vaccinated, were evaluated for determining the presence of specific IgM antibodies against measles virus. The samples were also evaluated for neutralizing antibodies against measles virus by a microneutralization test (MNT). By comparing the results of the ELISA with those of MNT, it was demonstrated that ELISA had a sensitivity and specificity of 100 and 92%, respectively. On the other hand, when the results obtained by our ELISA system were compared with those of an imported measles virus IgM ELISA kit (EIAgen; Adaltis Italia SPa, Bologna, Italy), a high level of agreement was shown (k = 0.926).
Authors:
F Roodbari; M H Roustai; A Mostafaie; H Soleimanjdahi; R Sarrami Foroshani; F Sabahi
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Clinical and diagnostic laboratory immunology     Volume:  10     ISSN:  1071-412X     ISO Abbreviation:  Clin. Diagn. Lab. Immunol.     Publication Date:  2003 May 
Date Detail:
Created Date:  2003-05-09     Completed Date:  2004-03-16     Revised Date:  2013-04-18    
Medline Journal Info:
Nlm Unique ID:  9421292     Medline TA:  Clin Diagn Lab Immunol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  439-42     Citation Subset:  IM    
Affiliation:
Department of Virology, Faculty of Medical Sciences, Tarbiat Modarres University, Tehran, Iran.
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MeSH Terms
Descriptor/Qualifier:
Animals
Antibodies, Anti-Idiotypic / biosynthesis,  diagnostic use
Antibodies, Viral / blood*,  immunology,  isolation & purification
Cercopithecus aethiops
Enzyme-Linked Immunosorbent Assay / methods*,  standards
Fluorescent Antibody Technique, Indirect
Humans
Immunoglobulin M / blood*,  immunology,  isolation & purification
Infant
Iran
Measles / diagnosis*
Measles virus / immunology*
Neutralization Tests
Rabbits
Sensitivity and Specificity
Vero Cells
Chemical
Reg. No./Substance:
0/Antibodies, Anti-Idiotypic; 0/Antibodies, Viral; 0/Immunoglobulin M
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