Document Detail


Development of enterovirus diagnostic assay system for human patients.
MedLine Citation:
PMID:  23336302     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
The coxsackieviruses B3 (CVB3) are members of the genus Enterovirus of the family Picornaviridae. They are the most common etiological agents of chronic myocarditis and dilated cardiomyopathy. However, there is still no effective diagnostic method for CVB3 in human patients. Here, we established a fast and accurate system to detect enterovirus (CVB3) in the sera of patients with viral myocarditis using a capsid-protein-specific peptide sequence. The peptide sequence was selected from the whole CVB3 capsid protein sequence by computationally predicting those fragments with high antigenicity and low hydrophobicity. Two of eight peptide sequences were selected and commercially synthesized. The synthesized peptides encoded either the VP2 or VP1 capsid protein and induced immunoglobulin G (IgG) antibody expression in immunized rabbits. Anti-VP2 and anti-VP1 sera detected the viral proteins extracted from CVB3-infected HeLa cells. The newly synthesized peptides successfully induced antibody production. These peptides, applied in an enzyme-linked immunosorbent assay (ELISA) system, detected anti-CVB3 antibodies in virus-infected mouse serum. The anti-CVB3 antibodies were detected by the new peptide-based ELISA system. Moreover, the ELISA system based on the VP2 peptide detected CVB3 infection of patients with positively identified CVB3 induced fulminant myocarditis. These results indicate that these new peptides specifically interact with anti-CVB3 IgG antibodies in mouse and human sera. This ELISA system should be applicable to the clinical diagnosis of enterovirus-induced myocarditis.
Authors:
Byung-Kwan Lim; Eun-Seon Ju; Dieu Hung Lao; Soo-Hyeon Yun; Yoo-Jung Lee; Duk-Kyung Kim; Eun-Seok Jeon
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2013-1-22
Journal Detail:
Title:  Microbiology and immunology     Volume:  -     ISSN:  1348-0421     ISO Abbreviation:  Microbiol. Immunol.     Publication Date:  2013 Jan 
Date Detail:
Created Date:  2013-1-22     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  7703966     Medline TA:  Microbiol Immunol     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
© 2013 The Societies and Blackwell Publishing Asia Pty Ltd.
Affiliation:
Division of Cardiology, Samsung Medical Center, Samsung Biomedical Research Institute, Sungkyunkwan University School of Medicine, 50 Irwon dong, Gangnam-gu, Seoul, 135-710, Korea.
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