| Development of an endocrine challenge test to investigate subfertility in ewes. | |
| | |
MedLine Citation:
|
PMID: 2686804 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Simultaneous or sequential injection of 250 ng gonadotrophin releasing hormone (GnRH) and 25 micrograms oestradiol benzoate, with luteinizing hormone (LH) measurements at 0, +20 min (after GnRH) and +16 h (after oestradiol), enabled investigation of the positive feedback effects on the hypothalamus and pituitary. Control ewes had pretreatment LH values of 3.1 +/- 1.2 ng/ml with an increment of 3.2 +/- 2.3 ng/ml 20 min after GnRH. Subfertile ewes, in spite of elevated pretreatment LH concentrations (15.8 +/- 9.5 ng/ml) in eight out of 10 ewes, had increments of 1.4-84 ng/ml after GnRH. Control ewes had LH increments of 3-75 ng/ml 16 h after oestradiol. Subfertile ewes with pretreatment LH concentrations less than 15 ng/ml also responded to oestradiol whereas those with initial LH concentrations 16-40 ng/ml had no further LH increment. Subsequent administration of 1000 iu pregnant mares' serum gonadotrophin (PMSG), with measurement of LH and oestradiol at 0, +24, +30, +48, +54, and +72 h, allowed assessment of ovarian response and hypothalamus-pituitary function. Five control ewes were sampled up to 30 h post-PMSG and only 1 had oestradiol concentrations greater than 10 pg/ml. Sampling up to 72 h in another five control ewes resulted in oestradiol concentrations greater than 10 pg/ml. Increments in LH concentration greater than 3 ng/ml were recorded in control and subfertile ewes with oestradiol concentrations greater than 10 pg/ml. The use of these endocrine challenge tests enabled positive diagnosis of abnormality on 8 out of 10 occasions.(ABSTRACT TRUNCATED AT 250 WORDS) |
| | |
Authors:
|
H Dobson; D A Davies |
Related Documents
:
|
6188824 - Endometrial protein secretion during early pregnancy in entire and ovariectomized ewes. 3507894 - Dopaminergic-like activity in toxic fescue alters prolactin but not growth hormone or t... 12072394 - Temporal requirements of thyroid hormones for seasonal changes in lh secretion. 3575334 - Prenatal androgen exposure and growth and secretion of growth hormone and prolactin in ... 9813444 - Evaluation of ovarian cysts following gnrh-a treatment in patients with polycystic ovar... 9000254 - Effect of arsenic (v) on the antioxidant defense system: in vitro oxidation of rat plas... |
Publication Detail:
|
Type: Journal Article |
Journal Detail:
|
Title: The British veterinary journal Volume: 145 ISSN: 0007-1935 ISO Abbreviation: Br. Vet. J. Publication Date: 1989 Nov-Dec |
Date Detail:
|
Created Date: 1990-01-24 Completed Date: 1990-01-24 Revised Date: 2003-11-14 |
Medline Journal Info:
|
Nlm Unique ID: 0372554 Medline TA: Br Vet J Country: ENGLAND |
Other Details:
|
Languages: eng Pagination: 523-30 Citation Subset: IM |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Animals Estradiol / blood, diagnostic use* Female Infertility, Female / blood, diagnosis, physiopathology, veterinary* Luteinizing Hormone / blood Pituitary Hormone-Releasing Hormones / diagnostic use* Sheep Sheep Diseases / blood, diagnosis*, physiopathology |
| Chemical | |
Reg. No./Substance:
|
0/Pituitary Hormone-Releasing Hormones; 50-28-2/Estradiol; 9002-67-9/Luteinizing Hormone |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Osteoporosis screening--radiogrammetry revisited.
Next Document: Dissociating performance and learning deficits on spatial navigation tasks in rats subjected to chol...