Document Detail


Development of electrical rhythmic activity in early embryonic cultured chick double-heart monitored optically with a voltage-sensitive dye.
MedLine Citation:
PMID:  4018408     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Double-hearted embryos were produced by whole-embryo culture of chick embryos which were microsurgically cut through the tissue of the anterior intestinal portal at the 1- to 6-somite developmental stage, at the time when the cardiac primordia have not yet fused in the bulboventricular region. The cultured embryos were removed from an incubator usually at the 7- to 10-somite stages of development, and then spontaneous electrical action potentials and/or contractions were optically recorded simultaneously from both the right and left half-hearts, using a 10 X 10- element photodiode matrix array together with a voltage-sensitive merocyanine-rhodanine dye (NK 2761). At the 7- to 8-somite stages, spontaneous action potentials were detected from bilateral prebeating half-hearts or sometimes from one half-heart. In each half-heart, the first spontaneous beating was often observed in the half-heart of the 9 somite embryos. In the beating half-hearts regular activity was always observed, while in the prebeating half-hearts at the 7- to 8-somite stages, both the regular and irregular rhythms of action potentials were detected, and the incidence of occurrence of regular activity significantly outnumbered that of the irregular rhythm. The heart rate in the left half-heart was faster than that in the right half-heart in the great majority of the prebeating and beating double-hearted embryos.
Authors:
T Yada; T Sakai; H Komuro; A Hirota; K Kamino
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Developmental biology     Volume:  110     ISSN:  0012-1606     ISO Abbreviation:  Dev. Biol.     Publication Date:  1985 Aug 
Date Detail:
Created Date:  1985-08-27     Completed Date:  1985-08-27     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0372762     Medline TA:  Dev Biol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  455-66     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Action Potentials
Animals
Chick Embryo / physiology*
Coloring Agents
Culture Techniques
Electrophysiology / methods
Heart / embryology*
Heart Conduction System / embryology
Heart Rate
Monitoring, Physiologic / methods*
Myocardial Contraction
Periodicity
Time Factors
Chemical
Reg. No./Substance:
0/Coloring Agents

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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