Document Detail


Development of the egg hatch assay for detection of anthelminthic resistance in human hookworms.
MedLine Citation:
PMID:  15885696     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Evidence of development and rapid spread of anthelminthic resistance in veterinary nematodes raises concern that the increasingly frequent treatments used in chemotherapy-based programmes to control human soil-transmitted helminths may select resistant worms. The aim of this study was to adapt, refine, and evaluate the Egg Hatch Assay (EHA) test, which has been used for veterinary nematodes, for field testing of benzimidazole (BZ) susceptibility/resistance in human hookworms. A second objective was to use this EHA to assess whether a population of worms resistant to mebendazole (MBZ) has built up in a sub-population of frequently treated children in Pemba Island. Stools from 470 school children enrolled in the first (Standard 1) and in the fifth (Standard 5) class were examined at baseline and at 21 days after treatment with 500 mg MBZ or placebo tablets. Standard 1 children had never received any MBZ treatment whilst Standard 5 children had received a total of 13 rounds of treatment. The EHA, involving culture of purified eggs with increasing drug concentrations showed that, for thiabendazole (TBZ), the mean ED(50)s (concentrations required to prevent 50% of the viable eggs from hatching) for all children at baseline were 0.079 microg/ml at 48h and 0.120 microg/ml at 72h (P<0.001). For MBZ, the mean ED(50)s for all children at baseline were 0.895 microg/ml at 48h and 1.50 microg/ml at 72h (P<0.001). For TBZ and for MBZ the ED(50) from Standard 1 were similar to those from Standard 5 children both at 48 and at 72h. At the follow-up for TBZ and for MBZ, there was no significant difference between the ED(50) from children who had received MBZ and children treated with placebo. In Pemba, TBZ ED(50) values of children non-exposed (Standard 1) and of children exposed (Standard 5) to MBZ treatment, and data from children treated with MBZ and placebo indicate that a drug-resistant worm population has not built up within treated individuals, and that periodic treatment has not yet selected for widespread BZ resistance, at least at the threshold detectable by the EHA in this study. However, ED(50) values for strains isolated from Mafia island, an area never exposed to BZ treatment were lower than for Pemba, suggesting lowered sensitivity of hookworm eggs recovered from Pembian children towards BZ.
Authors:
Marco Albonico; Victoria Wright; Mahdi Ramsan; Hamadi J Haji; Martin Taylor; Lorenzo Savioli; Quentin Bickle
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2005-04-26
Journal Detail:
Title:  International journal for parasitology     Volume:  35     ISSN:  0020-7519     ISO Abbreviation:  Int. J. Parasitol.     Publication Date:  2005 Jun 
Date Detail:
Created Date:  2005-05-31     Completed Date:  2006-01-23     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0314024     Medline TA:  Int J Parasitol     Country:  England    
Other Details:
Languages:  eng     Pagination:  803-11     Citation Subset:  IM    
Affiliation:
Ivo de Carneri Foundation, Via IV Marzo 14, 10122 Torino, Italy. albonico@tin.it
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MeSH Terms
Descriptor/Qualifier:
Ancylostomatoidea*
Animals
Anthelmintics* / therapeutic use
Benzimidazoles / therapeutic use
Child
Child, Preschool
Drug Resistance*
Eggs
Hookworm Infections / drug therapy*,  parasitology
Humans
Indian Ocean Islands
Levamisole / therapeutic use
Mebendazole / therapeutic use
Parasitology / methods
Chemical
Reg. No./Substance:
0/Anthelmintics; 0/Benzimidazoles; 14769-73-4/Levamisole; 31431-39-7/Mebendazole; 51-17-2/benzimidazole

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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