| Development of an efficient method for screening microorganisms by using symbiotic association between Nasutitermes takasagoensis and intestinal microorganisms. | |
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MedLine Citation:
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PMID: 17541579 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Screening method of microorganisms that utilized the symbiotic association between insect (Nasutitermes takasagoensis: Nt) and intestinal microorganisms was developed. The existence of desired microorganisms that grew by degrading difficult-to-degrade materials in the gut was detected using survivability of Nt as an indicator. The desired microorganisms were isolated from the survived Nt. It was thought that guts of Nt behave as continuous culture systems whereby microorganisms that cannot degrade diet components are washed out, whereas those that can degrade it are retained and concentrated in the gut. About 60% of Nt fed with phenol artificial diet (PAD) died within 7 days, while 4% of termites survived for 9 days. The structure of intestinal microorganisms of the survived Nt fed with PAD differed from the bacterial communities obtained from enrichment culture (which contained phenol) of wood-feeding Nt. Relatively high colonies (650-times) were detected in the gut of Nt fed on phenol artificial diet compared with those obtained when Nt was fed on wood. Seven denaturing gradient gel electrophoresis (DGGE) bands were detected from gut of wood-feeding Nt, whereas 11 DGGE-bands were detected from that of phenol-feeding Nt. Out of 11 DGGE-bands, 5 of them were sequenced, and bacterial species including phenol-degrading bacteria were identified. |
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Authors:
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Arata Hayashi; Hideki Aoyagi; Kazuhiko Kinjyo; Tsuyoshi Yoshimura; Hideo Tanaka |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2007-06-01 |
Journal Detail:
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Title: Applied microbiology and biotechnology Volume: 75 ISSN: 0175-7598 ISO Abbreviation: Appl. Microbiol. Biotechnol. Publication Date: 2007 Jul |
Date Detail:
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Created Date: 2007-06-22 Completed Date: 2007-09-18 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8406612 Medline TA: Appl Microbiol Biotechnol Country: Germany |
Other Details:
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Languages: eng Pagination: 1437-46 Citation Subset: IM |
Affiliation:
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Life Science and Bioengineering Laboratory, Graduate School of Life and Environmental Sciences, University of Tsukuba, Ibaraki, Tsukuba 305-8572, Japan. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Bacteria / genetics, metabolism* Base Sequence Intestines / microbiology* Isoptera / microbiology* Molecular Sequence Data Phenol / metabolism |
| Chemical | |
Reg. No./Substance:
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108-95-2/Phenol |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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