| Development and disintegration of phragmoplasts in living cultured cells of a GFP::TUA6 transgenic Arabidopsis thaliana plant. | |
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MedLine Citation:
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PMID: 12664275 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Cultured suspension cells of Arabidopsis thaliana that stably express a green-fluorescent protein-alpha-tubulin 6 fusion protein were used to follow the development and disintegration of phragmoplasts. The development and disintegration of phragmoplasts in the living cultured cells could be successively observed by detecting the green-fluorescent protein fluorescence of the microtubules. In the early telophase spindle, where two kinetochore groups and two daughter chromosome groups had completely separated from one another, fluorescence appeared in the interzone between the two chromosome groups. The fluorescent region was gradually condensed at the previous equator and increased in fluorescence intensity, and finally it formed the initial phragmoplast. The initial phragmoplast moved from the cell center towards the cell periphery, and it lost fluorescence at its center and became double rings in shape. The expansion orientation of the phragmoplast was not always the same as that of the future new cell wall before it came in contact with the cell wall. The phragmoplast did not usually come in contact with the cell wall simultaneously with its entire length. A portion of the phragmoplast which was earlier in contact with the cell wall disappeared earlier than other portions of the phragmoplast. The duration of contact between any portions of the phragmoplast and the plasma membrane of the cell wall was 15-30 min. The fluorescence intensity of the cytoplasm did not seem to be elevated by the disintegration of the strongly fluorescent phragmoplast. |
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Authors:
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K Ueda; S Sakaguchi; F Kumagai; S Hasezawa; H Quader; U Kristen |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Protoplasma Volume: 220 ISSN: 0033-183X ISO Abbreviation: Protoplasma Publication Date: 2003 Mar |
Date Detail:
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Created Date: 2003-03-28 Completed Date: 2004-01-16 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 9806853 Medline TA: Protoplasma Country: Austria |
Other Details:
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Languages: eng Pagination: 111-8 Citation Subset: IM |
Affiliation:
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Department of Biological Science, Nara Women's University, Nara. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Arabidopsis
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genetics,
metabolism,
ultrastructure* Cells, Cultured Cytoskeleton / ultrastructure Green Fluorescent Proteins Luminescent Proteins / genetics*, metabolism Metaphase Microscopy, Confocal Microtubules / ultrastructure Mitotic Spindle Apparatus / ultrastructure Plants, Genetically Modified / metabolism, ultrastructure Recombinant Fusion Proteins / analysis, metabolism Tubulin / analysis, genetics*, metabolism |
| Chemical | |
Reg. No./Substance:
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0/Luminescent Proteins; 0/Recombinant Fusion Proteins; 0/Tubulin; 147336-22-9/Green Fluorescent Proteins |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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