Document Detail


Development of a direct enzyme immunoassay of milk progesterone and its application to pregnancy diagnosis in cows.
MedLine Citation:
PMID:  6362087     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The purposes of this study were to develop an enzyme immunoassay (EIA) for determination of progesterone in unextracted whole milk and to apply it to pregnancy diagnosis. Paper fibers covalently coupled to antibody specifically and competitively bound 3H-progesterone and 11 alpha-hydroxyprogesterone hemisuccinate-horseradish peroxidase and were stable for 9 mo at -20 degrees C. The sensitivity, recovery, precision, and cross reactivity of the EIA and a radioimmunoassay (RIA) of milk progesterone were evaluated, and showed that this EIA was comparable to the RIA. Milk samples were collected once daily for one estrous cycle from ten lactating Holstein cows and the progesterone levels were determined by RIA. Milk progesterone in 70 samples measured by EIA were highly correlated (r = 0.90) with the values of RIA for the same samples. Milk samples for pregnancy diagnosis by EIA of milk progesterone were obtained daily from days 20 to 24 after 115 artificial inseminations of 85 lactating Holstein cows. Pregnancy diagnosis by EIA was confirmed by rectal palpation at 30 to 60 days after insemination or return to estrus. The accuracy based on single, two, three, four, and five consecutive samples was from 67.2 to 80.0%, 77.3 to 84.0%, 79.2 to 87.5%, 82.0 to 85.4%, and 85.4%, respectively, for pregnancy diagnosis; and from 95.0 to 98.3% for nonpregnancy.
Authors:
C F Chang; V L Estergreen
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Steroids     Volume:  41     ISSN:  0039-128X     ISO Abbreviation:  Steroids     Publication Date:  1983 Feb 
Date Detail:
Created Date:  1984-02-24     Completed Date:  1984-02-24     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0404536     Medline TA:  Steroids     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  173-95     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
Antibodies
Cattle
Drug Stability
Female
Immunoenzyme Techniques
Milk / analysis*
Pregnancy
Pregnancy, Animal*
Progesterone / analysis*
Radioimmunoassay / methods
Chemical
Reg. No./Substance:
0/Antibodies; 57-83-0/Progesterone

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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