Document Detail


Development of a derivatisation method for the analysis of aldehyde modified amino acid residues in proteins by Fourier transform mass spectrometry.
MedLine Citation:
PMID:  19166725     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A method was developed for the analysis of amino acids within bovine serum albumin (BSA) which had been modified by reaction with different enals. BSA was reacted with the aldehydes and the reaction products were stabilised by reaction with NaBH(4). The protein was then hydrolysed with 6N HCl and the hydrolysis products were analysed by liquid chromatography-mass spectrometry (LC-MS). The modified amino acids were derivatised with propylchloroformate. High resolution mass spectrometry carried out using an LTQ-Orbitrap instrument which was able to characterise a wide range of adducts. In addition double adducts were observed to be formed with 4-hydroxynonenal (HNE) and lysine or lysine+histidine. Qualitatively it was possible to consistently observe a pyridinium adduct formed between lysine and pentenal in human plasma from normal subjects.
Authors:
Mostafa Pournamdari; Ahmed Saadi; Elizabeth Ellis; Ruth Andrew; Brian Walker; David G Watson
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Publication Detail:
Type:  Journal Article     Date:  2008-12-06
Journal Detail:
Title:  Analytica chimica acta     Volume:  633     ISSN:  1873-4324     ISO Abbreviation:  Anal. Chim. Acta     Publication Date:  2009 Feb 
Date Detail:
Created Date:  2009-01-26     Completed Date:  2009-03-10     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0370534     Medline TA:  Anal Chim Acta     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  216-22     Citation Subset:  IM    
Affiliation:
Strathclyde Institute for Pharmacy and Biomedical Science (SIPBS), 27 Taylor Street, Glasgow G4 0NR, United Kingdom.
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MeSH Terms
Descriptor/Qualifier:
Aldehydes / analysis*,  chemistry
Amino Acids / analysis*
Animals
Blood Proteins / analysis*
Chromatography, Liquid
Fourier Analysis
Histidine / chemistry
Humans
Lysine / chemistry
Mass Spectrometry / methods*
Piperidines / chemistry
Pyridinium Compounds / chemistry
Schiff Bases / chemistry
Serum Albumin, Bovine / analysis*
Chemical
Reg. No./Substance:
0/Aldehydes; 0/Amino Acids; 0/Blood Proteins; 0/Piperidines; 0/Pyridinium Compounds; 0/Schiff Bases; 0/Serum Albumin, Bovine; 29343-52-0/4-hydroxy-2-nonenal; 56-87-1/Lysine; 71-00-1/Histidine

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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