Document Detail


Development of a cytochrome c-based screen-printed biosensor for the determination of the antioxidant capacity of orange juices.
MedLine Citation:
PMID:  19447685     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
This paper describes the development of an amperometric cytochrome c (cyt c)-based biosensor and its later application to the quantification of the scavenging capacity of antioxidants. The enzymatic biosensor was constructed by covalently co-immobilizing both cyt c and XOD on a mercaptoundecanol/mercaptoundecanoic acid (MU/MUA) mixed self-assembled monolayer (SAM)-modified screen-printed gold electrode. The applicability of this method was shown by analyzing the antioxidant capacity of pure substances, such as ascorbic acid and Trolox, and natural sources of antioxidants, particularly 5 orange juices.
Authors:
Montserrat Cortina-Puig; Xavier Muñoz-Berbel; Regis Rouillon; Carole Calas-Blanchard; Jean-Louis Marty
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2009-05-04
Journal Detail:
Title:  Bioelectrochemistry (Amsterdam, Netherlands)     Volume:  76     ISSN:  1878-562X     ISO Abbreviation:  Bioelectrochemistry     Publication Date:  2009 Sep 
Date Detail:
Created Date:  2009-08-18     Completed Date:  2009-12-01     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  100953583     Medline TA:  Bioelectrochemistry     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  76-80     Citation Subset:  IM    
Affiliation:
IMAGES EA 4218, Université de Perpignan Via Domitia, 52 Avenue Paul Alduy, 66860 Perpignan Cedex, France.
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MeSH Terms
Descriptor/Qualifier:
Animals
Antioxidants / analysis,  chemistry*,  metabolism
Beverages*
Biosensing Techniques / methods*
Cattle
Citrus sinensis / chemistry*
Cytochromes c / chemistry*,  metabolism
Electrochemistry
Electrodes
Enzymes, Immobilized / chemistry,  metabolism
Gold / chemistry,  metabolism
Horses
Printing*
Superoxides / chemistry,  metabolism
Xanthine Oxidase / chemistry,  metabolism
Chemical
Reg. No./Substance:
0/Antioxidants; 0/Enzymes, Immobilized; 11062-77-4/Superoxides; 7440-57-5/Gold; 9007-43-6/Cytochromes c; EC 1.17.3.2/Xanthine Oxidase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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