Document Detail


Development of competitive mRNA PCR for the quantification of interleukin-6-responsive junB oncogene expression.
MedLine Citation:
PMID:  9591138     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The transcription factor junB belongs to the jun family of protooncogenes. The appearance of junB mRNA in hepatic cells is an extremely early and sensitive marker of the action of proinflammatory cytokines including interleukin-6. In this study, a competitive reverse transcription (RT)-PCR assay has been developed that is suitable for the quantitative determination of junB mRNA expression. This nonisotopic assay compared to other methods (e.g., Northern blot) is a fast and convenient way to determine the expression of the junB gene and thus the immediate concentration- and time-dependent action of interleukin-6. Because interleukin-6 and interleukin-6-type cytokines play a highly important regulatory role in various pathophysiologically important processes, such as hepatic acute-phase reaction, the quantitative assay of junB mRNA completes the scale of laboratory approaches in inflammation and among other pathological conditions.
Authors:
P Igaz; G Fejér; C Szalai; S Tóth; A Falus
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  BioTechniques     Volume:  24     ISSN:  0736-6205     ISO Abbreviation:  BioTechniques     Publication Date:  1998 May 
Date Detail:
Created Date:  1998-07-21     Completed Date:  1998-07-21     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8306785     Medline TA:  Biotechniques     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  854-60     Citation Subset:  IM    
Affiliation:
Semmelweis University Medical School, Budapest, Hungary.
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MeSH Terms
Descriptor/Qualifier:
Base Sequence
Carcinoma, Hepatocellular
DNA Primers
Gene Expression Regulation, Neoplastic / drug effects*
Genes, jun / drug effects*
Humans
Interleukin-6 / pharmacology*
Molecular Sequence Data
Polymerase Chain Reaction / methods*,  standards
RNA, Messenger / genetics*
Transcription Factors / genetics
Tumor Cells, Cultured
Chemical
Reg. No./Substance:
0/DNA Primers; 0/Interleukin-6; 0/RNA, Messenger; 0/Transcription Factors

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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