| Development of competence of Haemophilus influenzae. | |
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MedLine Citation:
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PMID: 5294817 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Spencer, Hugh T. (The Johns Hopkins University School of Hygiene and Public Health, Baltimore, Md.), and Roger M. Herriott. Development of competence of Haemophilus influenzae. J. Bacteriol. 90:911-920. 1965.-A chemically defined nongrowth medium was developed for the induction of competence of Haemophilus influenzae by a stepdown procedure. Cells grown logarithmically in Heart Infusion Broth became competent after being transferred to a medium which consisted of amino acids, sodium fumarate, and inorganic salts. Chloramphenicol (2 mug/ml) or l-valine (1 mug/ml) in the nongrowth medium inhibited development of competence. The inhibitory action of l-valine was reversed by comparable concentrations of l-isoleucine. Kinetic studies of the development of competence showed a variable capacity of competent cells to take up deoxyribonucleic acid and reaffirmed earlier findings that competence was not transmissible in H. influenzae. Addition of nicotinamide adenine dinucleotide, thiamine, calcium pantothenate, uracil, and hypoxanthine to the medium for competence resulted in a minimal growth medium in which reduced levels of competence were developed. |
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Authors:
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H T Spencer; R M Herriott |
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Publication Detail:
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Type: In Vitro; Journal Article |
Journal Detail:
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Title: Journal of bacteriology Volume: 90 ISSN: 0021-9193 ISO Abbreviation: J. Bacteriol. Publication Date: 1965 Oct |
Date Detail:
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Created Date: 1966-02-17 Completed Date: 1966-02-17 Revised Date: 2009-11-18 |
Medline Journal Info:
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Nlm Unique ID: 2985120R Medline TA: J Bacteriol Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 911-20 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Amino Acids
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pharmacology Culture Media DNA, Bacterial* Genetics Haemophilus influenzae* / growth & development* Leucine / pharmacology Uracil / pharmacology Valine / pharmacology |
| Chemical | |
Reg. No./Substance:
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0/Amino Acids; 0/Culture Media; 0/DNA, Bacterial; 61-90-5/Leucine; 66-22-8/Uracil; 7004-03-7/Valine |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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