| Development and comparison of SYBR Green quantitative real-time PCR assays for detection and enumeration of sulfate-reducing bacteria in stored swine manure. | |
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MedLine Citation:
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PMID: 19120660 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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AIMS: To develop and evaluate primer sets targeted to the dissimilatory sulfite reductase gene (dsrA) for use in quantitative real-time PCR detection of sulfate-reducing bacteria (SRB) in stored swine manure. METHODS AND RESULTS: Degenerate primer sets were developed to detect SRB in stored swine manure. These were compared with a previously reported primer set, DSR1F+ and DSR-R, for their coverage and ability to detect SRB communities in stored swine manure. Sequenced clones were most similar to Desulfovibrio sp. and Desulfobulbus sp., and these SRB populations differed within different manure ecosystems. Sulfur content of swine diets was shown to affect the population of Desulfobulbus-like Group 1 SRB in manure. CONCLUSIONS: The newly developed assays were able to enumerate and discern different groups of SRB, and suggest a richly diverse and as yet undescribed population of SRB in swine manure. SIGNIFICANCE AND IMPACT OF THE STUDY: The PCR assays described here provide improved and efficient molecular tools for quantitative detection of SRB populations. This is the first study to show population shifts of SRB in swine manure, which are a result of either the effects of swine diets or the maturity of the manure ecosystem. |
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Authors:
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C Spence; T R Whitehead; M A Cotta |
Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Journal of applied microbiology Volume: 105 ISSN: 1365-2672 ISO Abbreviation: J. Appl. Microbiol. Publication Date: 2008 Dec |
Date Detail:
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Created Date: 2009-01-05 Completed Date: 2009-10-09 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9706280 Medline TA: J Appl Microbiol Country: England |
Other Details:
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Languages: eng Pagination: 2143-52 Citation Subset: IM |
Affiliation:
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Fermentation Biotechnology Research Unit, National Center for Agricultural Research, USDA, Agricultural Research Service, Peoria, IL 61604, USA. cheryl.spence@ars.usda.gov |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals DNA Primers / genetics DNA, Bacterial / analysis Manure / microbiology* Polymerase Chain Reaction / methods* RNA, Ribosomal, 16S / analysis Sequence Analysis, DNA Sulfur-Reducing Bacteria / genetics, isolation & purification* Swine |
| Chemical | |
Reg. No./Substance:
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0/DNA Primers; 0/DNA, Bacterial; 0/Manure; 0/RNA, Ribosomal, 16S |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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