Document Detail


Development of a chromatographic fingerprint for the chloroform extracts of Ganoderma lucidum by HPLC and LC-MS.
MedLine Citation:
PMID:  18337046     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A new high-performance liquid chromatographic (HPLC) fingerprinting method was developed for the quality control of Ganoderma lucidum. Twenty-nine batches obtained from three different origins in China were used to establish the fingerprint. The constituents of these samples were separated with a Kromasil C(18) column (250 mm x 4.6 mm, 5 microm) by linear gradient elution using water-acetic acid (100:0.1, v/v) and acetonitrile as mobile phase components at a flow rate of 0.8 ml/min and detector wavelength at 254 nm. Mean chromatograms and correlation coefficients of samples were calculated by the software "Similarity Evaluation System for Chromatographic Fingerprint of TCM". There were 19 common peaks in this fingerprint. Eleven of these common peaks were tentatively identified with reference to literature data based on their liquid chromatography-electrospray ionization-tandem mass spectrometry (LC-ESI-MS) and UV data. This profile was then successfully used to identify and assess the differences among samples from various origins with the aid of similarity analysis. The diverse similarities among different samples indicated that the quality of G. lucidum was not stable and the products from different areas were inconsistent. All results showed that the developed fingerprint assay was specific and could further serve for quality identification and comprehensive evaluation of G. lucidum.
Authors:
Yi Chen; Yan Yan; Ming-Yong Xie; Shao-Ping Nie; Wei Liu; Xiao-Feng Gong; Yuan-Xing Wang
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2008-02-06
Journal Detail:
Title:  Journal of pharmaceutical and biomedical analysis     Volume:  47     ISSN:  0731-7085     ISO Abbreviation:  J Pharm Biomed Anal     Publication Date:  2008 Jul 
Date Detail:
Created Date:  2008-05-27     Completed Date:  2008-09-09     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8309336     Medline TA:  J Pharm Biomed Anal     Country:  England    
Other Details:
Languages:  eng     Pagination:  469-77     Citation Subset:  IM    
Affiliation:
State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, People's Republic of China.
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MeSH Terms
Descriptor/Qualifier:
Chromatography, High Pressure Liquid / methods*
Plant Extracts / analysis*
Reference Standards
Reishi / chemistry*
Reproducibility of Results
Spectrometry, Mass, Electrospray Ionization / methods*
Chemical
Reg. No./Substance:
0/Plant Extracts

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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