Document Detail


Development of chloroplast microsatellite markers for phylogenetic analysis in Brassicaceae.
MedLine Citation:
PMID:  23134603     Owner:  NLM     Status:  In-Data-Review    
Abstract/OtherAbstract:
By employing in silico tools, we devised new chloroplast microsatellite primers for inferring phylogenetic relationships within Brassicaceae. Microsatellite repeats were scanned in 12 chloroplast genomes of Brassicaceae, regions flanking these repeats were aligned and 19 universal primers were designed. Fifteen of these primer pairs are predicted to yield polymorphic amplicons, that are more or less evenly distributed throughout the chloroplast genomes. Finally, using PCR, we have validated three primer pairs on a limited 'test set' of plants, different from those used in computational analysis.
Authors:
Praveen Awasthi; Irshad Ahmad; Sumit G Gandhi; Yashbir S Bedi
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Acta biologica Hungarica     Volume:  63     ISSN:  0236-5383     ISO Abbreviation:  Acta. Biol. Hung.     Publication Date:  2012 Dec 
Date Detail:
Created Date:  2012-11-08     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8404358     Medline TA:  Acta Biol Hung     Country:  Hungary    
Other Details:
Languages:  eng     Pagination:  463-73     Citation Subset:  IM    
Affiliation:
Council of Scientific and Industrial Research Plant Biotechnology Division, Indian Institute of Integrative Medicine Canal Road Jammu 180 001 India.
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