Document Detail


Development and characterization of three new diploid cell lines from Labeo rohita (Ham.).
MedLine Citation:
PMID:  20730759     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Development of cell lines from fish for identifying the pathogenesis of viral diseases and for vaccine production against viral and bacterial diseases is imperative where they are of commercial importance. Three new diploid fish cell lines (RF, RH, and RSB) were developed from fin, heart, and swim bladder of an Indian major carp, Labeo rohita, commonly called Rohu. All the cell lines were optimally maintained at 28 degrees C in Leibovitz-15 medium supplemented with 10% FBS. The propagation of RH and RSB cells was serum dependent, with a low plating efficiency (<16%), whereas RF cells showed 20% efficiency. The cytogenetic analysis revealed a diploid count of 50 chromosomes. The cells of RF and RSB were found to be epithelial, where as the cells of RH were mostly fibroblastic. The viability of the RF, RH, and RSB cell lines was 75, 70 and 72%, respectively after 6 months of storage in liquid nitrogen. The origin of the cell lines was confirmed by the amplification of 496 and 655 bp fragments of 16S rRNA and Cytochrome Oxidase Subunit I (COI) of mtDNA. The new cell lines would facilitate viral disease diagnosis and genomic studies.
Authors:
Wazir S Lakra; T Raja Swaminathan; Gaurav Rathore; Mukunda Goswami; Kamalendra Yadav; Smita Kapoor
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Biotechnology progress     Volume:  26     ISSN:  1520-6033     ISO Abbreviation:  Biotechnol. Prog.     Publication Date:    2010 Jul-Aug
Date Detail:
Created Date:  2010-08-23     Completed Date:  2010-12-08     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8506292     Medline TA:  Biotechnol Prog     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1008-13     Citation Subset:  IM    
Copyright Information:
(c) 2010 American Institute of Chemical Engineers
Affiliation:
National Bureau of Fish Genetic Resources, Dilkusha, Lucknow, India. wslakra@gmail.com
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MeSH Terms
Descriptor/Qualifier:
Animals
Carps
Cell Culture Techniques / methods*
Cell Line
Diploidy*

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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