| Development and characterization of a novel human Waldenström Macroglobulinemia cell line (RPCI-WM1; Roswell Park Cancer Institute-Waldenström Macroglobulinemia 1). | |
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MedLine Citation:
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PMID: 22812491 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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Abstract Understanding the biology of Waldenström Macroglobulinemia is hindered by a lack of preclinical models. We report a novel cell line, RPCI-WM1, from a patient treated for WM. The cell line secreted human IgM (hIgM) with k-light chain restriction identical to the primary tumor. The cell line has a modal chromosomal number of 46 and harbors chromosomal changes such as deletion of 6q21, monoallelic deletion of 9p21 (CDKN2A), 13q14 (RB1) and 18q21 (BCL-2) with a consistent amplification of 14q32 (IgH) identical to its founding tumor sample. Clonal relationship was confirmed by identical CDR3 length, and single nucleotide polymorphisms as well as a matching IgH sequence of the cell line and founding tumor. Both also harbor a heterozygous, non-synonymous mutation at amino acid 265 in MYD88 gene (L265P). The cell line expresses most of the cell surface markers present on the parent cells. Over all, RPCI-WM1 represents a valuable model to study WM. |
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Authors:
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Kasyapa S Chitta; Aneel Paulus; Sikander Ailawadhi; Barbara A Foster; Michael T Moser; Petr Starostik; Aisha Masood; Taimur Sher; Kena C Miller; Dan M Iancu; Jeffrey Conroy; Norma J Nowak; Sheila N Sait; David A Personett; Morton Coleman; Richard R Furman; Peter Martin; Stephen M Ansell; Kelvin Lee; Asher A Chanan-Khan |
Publication Detail:
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Type: JOURNAL ARTICLE Date: 2012-7-19 |
Journal Detail:
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Title: Leukemia & lymphoma Volume: - ISSN: 1029-2403 ISO Abbreviation: - Publication Date: 2012 Jul |
Date Detail:
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Created Date: 2012-7-20 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9007422 Medline TA: Leuk Lymphoma Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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