Document Detail


Development and characterization of a novel human Waldenström macroglobulinemia cell line: RPCI-WM1, Roswell Park Cancer Institute - Waldenström Macroglobulinemia 1.
MedLine Citation:
PMID:  22812491     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Understanding the biology of Waldenström macroglobulinemia is hindered by a lack of preclinical models. We report a novel cell line, RPCI-WM1, from a patient treated for WM. The cell line secretes human immunoglobulin M (h-IgM) with κ-light chain restriction identical to the primary tumor. The cell line has a modal chromosomal number of 46 and harbors chromosomal changes such as deletion of 6q21, monoallelic deletion of 9p21 (CDKN2A), 13q14 (RB1) and 18q21 (BCL-2), with a consistent amplification of 14q32 (immunoglobulin heavy chain; IgH) identical to its founding tumor sample. The clonal relationship is confirmed by identical CDR3 length and single nucleotide polymorphisms as well as a matching IgH sequence of the cell line and founding tumor. Both also harbor a heterozygous, non-synonymous mutation at amino acid 265 in the MYD88 gene (L265P). The cell line expresses most of the cell surface markers present on the parent cells. Overall, RPCI-WM1 represents a valuable model to study Waldenström macroglobulinemia.
Authors:
Kasyapa S Chitta; Aneel Paulus; Sikander Ailawadhi; Barbara A Foster; Michael T Moser; Petr Starostik; Aisha Masood; Taimur Sher; Kena C Miller; Dan M Iancu; Jeffrey Conroy; Norma J Nowak; Sheila N Sait; David A Personett; Morton Coleman; Richard R Furman; Peter Martin; Stephen M Ansell; Kelvin Lee; Asher A Chanan-Khan
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't     Date:  2012-08-27
Journal Detail:
Title:  Leukemia & lymphoma     Volume:  54     ISSN:  1029-2403     ISO Abbreviation:  Leuk. Lymphoma     Publication Date:  2013 Feb 
Date Detail:
Created Date:  2013-01-07     Completed Date:  2013-06-17     Revised Date:  2014-04-08    
Medline Journal Info:
Nlm Unique ID:  9007422     Medline TA:  Leuk Lymphoma     Country:  England    
Other Details:
Languages:  eng     Pagination:  387-96     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
Base Sequence
Cell Line, Tumor*
Cytogenetic Analysis
Disease Models, Animal
Female
Humans
Immunoglobulin Heavy Chains / chemistry,  genetics
Immunoglobulin M / biosynthesis,  genetics
Immunoglobulin kappa-Chains / genetics,  metabolism
Immunophenotyping
Mice
Middle Aged
Molecular Sequence Data
Mutation
Myeloid Differentiation Factor 88 / genetics
Polymorphism, Single Nucleotide
Sequence Alignment
Transplantation, Heterologous
Waldenstrom Macroglobulinemia / genetics*,  metabolism*,  pathology
Grant Support
ID/Acronym/Agency:
P30 CA016056/CA/NCI NIH HHS; R01 CA097243/CA/NCI NIH HHS; R01 CA121044-01/CA/NCI NIH HHS
Chemical
Reg. No./Substance:
0/Immunoglobulin Heavy Chains; 0/Immunoglobulin M; 0/Immunoglobulin kappa-Chains; 0/Myeloid Differentiation Factor 88
Comments/Corrections
Comment In:
Leuk Lymphoma. 2013 Feb;54(2):224-6   [PMID:  22950966 ]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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