| Development and characterization of normal colonic epithelial cell lines derived from normal mucosa of patients with colon cancer. | |
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MedLine Citation:
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PMID: 7840378 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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BACKGROUND: Researchers have tried for at least 20 years to develop a normal human colonic cell line suitable for in vitro studies of human colonic diseases. We report a breakthrough development of two normal colon-derived cell lines. They are designated NCM356 and NCM425. MATERIALS AND METHODS: The cells were collected from the histologically normal colonic margin of patients undergoing resection for colon adenocarcinomas and grown in culture. RESULTS: Since NCM356 and NCM425 have now been subcultured 22 and 19 times, each has undergone more than 40 population doublings. Neither cell line has shown evidence of terminal differentiation. Immunohistochemical characterization studies demonstrated that they are epithelial cells. They variably expressed subsets of other markers, including tumor markers, but did not grow in soft agar. NCM356 did not form tumors, whereas NCM425 was tumorigenic in immunodeficient mice. CONCLUSION: These two cell lines represent the first successful in vitro culture of human colonocytes derived from normal mucosa. NCM356 is closer to normal, but seems to represent an early stage of cell transformation, possibly correlated with immortalization. In contrast, in vitro culture of the NCM425 cell line appears to have selected for later progression to malignancy. These lines are important resources for studying colon cancer and the physiology of intestinal cells. |
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Authors:
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J S Stauffer; L A Manzano; G C Balch; R L Merriman; L R Tanzer; M P Moyer |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: American journal of surgery Volume: 169 ISSN: 0002-9610 ISO Abbreviation: Am. J. Surg. Publication Date: 1995 Feb |
Date Detail:
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Created Date: 1995-02-28 Completed Date: 1995-02-28 Revised Date: 2004-11-17 |
Medline Journal Info:
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Nlm Unique ID: 0370473 Medline TA: Am J Surg Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 190-5; discussion 195-6 Citation Subset: AIM; IM |
Affiliation:
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Department of Surgery, University of Texas Health Science Center at San Antonio 78284-7842. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Adenocarcinoma
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pathology Aged Animals Cell Division Cell Line* Cell Line, Transformed Cell Transformation, Neoplastic / pathology Colon / cytology*, ultrastructure Colonic Neoplasms / pathology* DNA / analysis Epithelial Cells Flow Cytometry Humans Intestinal Mucosa / cytology*, ultrastructure Male Mice Mice, Inbred Strains Middle Aged |
| Chemical | |
Reg. No./Substance:
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9007-49-2/DNA |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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