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Development and characterization of monoclonal antibody against human FcR γ chain.
MedLine Citation:
PMID:  21722532     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
AIM: The current study was aimed to generate mouse monoclonal antibodies (mAbs) specific for Fc receptor common γ chain (FcRγ), which serves a critical signaling molecule for numerous human immune receptors that play essential roles in human immune responses. METHODS: Two BALB/c mice were immunized with the synthesized polypeptides containing 19 residues at the carboxyl terminus of human FcR γ chain. The spleen cells from the immunized mice were fused with myeloma Sp2/0 cells. ELISA was used to screen mAbs and the positive clones were selected for further characterization with Western blot and flow cytometry assays. RESULTS: After screen, there hybridoma cell lines which secreted mAbs specifically recognized the FcR γ chain were obtained and named as 5B6, 7D3 and 8D4. The 7D3 mAb had the best quality and it could recognize the FcR γ chain in vivo and in vitro with 2.5 μg/mL. CONCLUSION: We have generated the FcRγ-specific mAbs, which may serve as a valuable tool in the functional studies of FcRγ associated immune receptors.
Authors:
Kun Qian; Wen-Jie Jin; Hong-Xia Shao; Jian-Ming Wu; Ai-Jian Qin
Publication Detail:
Type:  JOURNAL ARTICLE    
Journal Detail:
Title:  Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology     Volume:  27     ISSN:  1007-8738     ISO Abbreviation:  -     Publication Date:  2011 Jul 
Date Detail:
Created Date:  2011-7-4     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101139110     Medline TA:  Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi     Country:  -    
Other Details:
Languages:  CHI     Pagination:  773-776     Citation Subset:  -    
Affiliation:
Key Lab. of Jiangsu Preventive Veterinary Medicine, Yangzhou University, Yangzhou 255009, China.
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