| Development and characterization of monoclonal antibodies to lymphocyte cell surface antigens of the rhesus monkey. | |
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MedLine Citation:
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PMID: 1604487 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Three monoclonal antibodies directed against rhesus lymphocyte cell surface antigens are described. A pan-T mAb, T64, and a T suppressor mAb, T35, showed phenotypic and functional specificity for both human and rhesus cells. In contrast, a third mAb, N42, identifying natural killer cells in rhesus peripheral blood leukocytes, was not crossreactive with the corresponding homologous human cells. N42 reacted with the same cells identified by Leu 11a and Leu 11b in rhesus PBL and in a functional assay decreased NK activity by 80%. N42 precipitated a 50KD protein from rhesus PBL lysates and was reactive with the Fc receptor domain of the NK cell. The T-S functional activity of cells reactive with mAb T35 was demonstrated in a pokeweed-mitogen-driven system for Ig synthesis: removal of the T35 positive cells by complement-mediated lysis led to an enhanced production of Ig by rhesus PBL, and the addition of T35 positive cells to a culture of T helper and B cells resulted in a reduction of this response. T35 was determined to be an IgG2a immunoglobulin and precipitated a 34KD protein from rhesus cell lysates. An IgM immunoglobulin, mAb T64 delineated all T lymphocytes, inhibited E-rosette formation, interfered with the proliferation of cells stimulated with mitogens, and precipitated a 52KD membrane protein. The potential utilization of these mAbs in vivo for organ or tissue transplantation in the rhesus monkey is discussed. |
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Authors:
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N Gengozian; R A Good; S M Fu |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Transplantation Volume: 53 ISSN: 0041-1337 ISO Abbreviation: Transplantation Publication Date: 1992 Jun |
Date Detail:
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Created Date: 1992-07-14 Completed Date: 1992-07-14 Revised Date: 2007-11-14 |
Medline Journal Info:
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Nlm Unique ID: 0132144 Medline TA: Transplantation Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 1306-12 Citation Subset: IM |
Affiliation:
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Department of Pediatrics, All Children's Hospital, St. Petersburg, Florida 33701. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Antibodies, Monoclonal / chemistry, immunology* Antibody Formation Antigens, Surface / immunology* Binding, Competitive Female Killer Cells, Natural / immunology Lymphocytes / immunology* Macaca mulatta / immunology* Male T-Lymphocyte Subsets / immunology |
| Grant Support | |
ID/Acronym/Agency:
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AG05628/AG/NIA NIH HHS; AI22360/AI/NIAID NIH HHS; CA34546/CA/NCI NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Antibodies, Monoclonal; 0/Antigens, Surface |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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