Document Detail


Development and characterization of SV40 immortalized rat parotid acinar cell lines.
MedLine Citation:
PMID:  9542637     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Rat parotid salivary gland acinar cells were transfected by CaPO4 precipitation using a plasmid containing a replication-defective simian virus (SV40) genome. Out of 30 clonal cell lines, 2 were shown to have moderate to high levels of cytodifferentiation and salivary gland acinar cell function. Functional studies with the two cell lines indicated that the beta-adrenergic agonist (isoproterenol), vasoactive intestinal peptide prostaglandin E1, and forskolin were effective activators of intracellular cyclic adenosine 3':5'-cyclic monophosphate production. Phenylephrine, carbamylcholine, and UTP were effective in increasing inositol phosphate production and intracellular free calcium levels, whereas substance P was without affect. Utilizing indirect immunofluorescence analysis, both cell lines were shown to express the SV40 large T antigen. Electron microscopic evaluation documented moderate to high levels of cytodifferentiation with the maintenance of tripartite junctional complexes, cellular polarization, and presence of moderate amounts of secretory granules and rough endoplasmic reticulum. The two cell lines had doubling times of 22 and 36 h, respectively.
Authors:
D O Quissell; K A Barzen; R S Redman; J M Camden; J T Turner
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, Non-P.H.S.; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  In vitro cellular & developmental biology. Animal     Volume:  34     ISSN:  1071-2690     ISO Abbreviation:  In Vitro Cell. Dev. Biol. Anim.     Publication Date:  1998 Jan 
Date Detail:
Created Date:  1998-05-26     Completed Date:  1998-05-26     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  9418515     Medline TA:  In Vitro Cell Dev Biol Anim     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  58-67     Citation Subset:  IM    
Affiliation:
Department of Basic Sciences and Oral Research, School of Dentistry, University of Colorado Health Sciences Center, Denver 80262, USA.
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MeSH Terms
Descriptor/Qualifier:
3T3 Cells
Animals
Cell Line, Transformed*
Male
Mice
Parotid Gland / cytology*
Plasmids
Rats
Rats, Sprague-Dawley
Simian virus 40*
Transfection
Grant Support
ID/Acronym/Agency:
DE07389/DE/NIDCR NIH HHS; DE11153/DE/NIDCR NIH HHS

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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