Document Detail


Development and characterization of DehaloR^2, a novel anaerobic microbial consortium performing rapid dechlorination of TCE to ethene.
MedLine Citation:
PMID:  21667274     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A novel anaerobic consortium, named DehaloR^2, that performs rapid and complete reductive dechlorination of trichloroethene (TCE) to ethene is described. DehaloR^2 was developed from estuarine sediment from the Back River of the Chesapeake Bay and has been stably maintained in the laboratory for over 2 years. Initial sediment microcosms showed incomplete reduction of TCE to DCE with a ratio of trans- to cis- isomers of 1.67. However, complete reduction to ethene was achieved within 10 days after transfer of the consortium to sediment-free media and was accompanied by a shift to cis-DCE as the prevailing intermediate metabolite. The microbial community shifted from dominance of the Proteobacterial phylum in the sediment to Firmicutes and Chloroflexi in DehaloR^2, containing the genera Acetobacterium, Clostridium, and the dechlorinators Dehalococcoides. Also present were Spirochaetes, possible acetogens, and Geobacter which encompass previously described dechlorinators. Rates of TCE to ethene reductive dechlorination reached 2.83 mM Cl- d(-1) in batch bottles with a Dehalococcoides sp. density of 1.54E+11 gene copies per liter, comparing favorably to other enrichment cultures described in the literature and identifying DehaloR^2 as a promising consortium for use in bioremediation of chlorinated ethene-impacted environments.
Authors:
Michal Ziv-El; Anca G Delgado; Ying Yao; Dae-Wook Kang; Katherine G Nelson; Rolf U Halden; Rosa Krajmalnik-Brown
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Publication Detail:
Type:  Evaluation Studies; Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't     Date:  2011-06-12
Journal Detail:
Title:  Applied microbiology and biotechnology     Volume:  92     ISSN:  1432-0614     ISO Abbreviation:  Appl. Microbiol. Biotechnol.     Publication Date:  2011 Dec 
Date Detail:
Created Date:  2011-11-09     Completed Date:  2012-02-15     Revised Date:  2012-07-26    
Medline Journal Info:
Nlm Unique ID:  8406612     Medline TA:  Appl Microbiol Biotechnol     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  1063-71     Citation Subset:  IM    
Affiliation:
Swette Center for Environmental Biotechnology, Biodesign Institute at Arizona State University, PO Box 875001, Tempe, AZ 85287-5001, USA.
Data Bank Information
Bank Name/Acc. No.:
GENBANK/HQ012835;  HQ012836;  HQ012837;  HQ012838;  HQ012839;  HQ012840;  HQ012841;  HQ012842;  HQ012843
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MeSH Terms
Descriptor/Qualifier:
Anaerobiosis
Bacteria / classification,  genetics,  isolation & purification,  metabolism*
Biodegradation, Environmental
Environmental Remediation / methods*
Ethylenes / chemistry,  metabolism*
Geologic Sediments / microbiology*
Microbial Consortia*
Molecular Sequence Data
Oxidation-Reduction
Phylogeny
Trichloroethylene / chemistry,  metabolism*
Grant Support
ID/Acronym/Agency:
R01ES015445/ES/NIEHS NIH HHS
Chemical
Reg. No./Substance:
0/Ethylenes; 74-85-1/ethylene; 79-01-6/Trichloroethylene
Comments/Corrections
Erratum In:
Appl Microbiol Biotechnol. 2012 Jul;95(1):273-4

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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