| Development and characteristics of a human cell assay for screening agents for melanoma prevention. | |
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MedLine Citation:
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PMID: 17235241 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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This paper describes the development and initial evaluation of a human cell assay to identify potentially efficacious agents for preventing melanoma. Four human cell lines were used: normal melanocytes, a radial growth-phase-like melanoma cell line (WM3211), a vertical growth-phase-like melanoma cell line (Lu1205), and 83-2c, a cell strain cloned from metastatic melanoma. Four endpoints were evaluated in ultraviolet B-treated cells: annexin V, human leukocyte antigen-DR; E-cadherin, and N-cadherin. Annexin V was induced by nimesulide, 4-hydroxyphenylretinamide, and difluoromethylornithine in ultraviolet-B-treated radial growth-phase-like melanoma cells. None of the agents inhibited human leukocyte antigen-DR expression in ultraviolet-B-treated radial growth-phase-like melanoma cells, the only cells that strongly expressed human leukocyte antigen-DR. E-cadherin was overexpressed only in radial growth-phase-like melanoma cells relative to melanocytes, and ultraviolet B exposure dramatically reduced this expression. E-cadherin was only induced by difluoromethylornithine in ultraviolet-B-treated radial growth-phase-like melanoma cells. N-cadherin was over- expressed in all melanoma cell lines relative to melanocytes. In this study, all candidate preventive agents inhibited N-cadherin in ultraviolet B-treated radial growth-phase-like melanoma cells. Four agents inhibited N-cadherin in ultraviolet B-treated vertical growth-phase-like melanoma cells. The mean ratios of N-cadherin to E-cadherin levels and specific endpoint responses for both the radial growth-phase-like melanoma and vertical growth-phase-like melanoma cells were used to rank the agents. Agents were evaluated at clinically relevant concentrations. The rankings were difluoromethylornithine>4-hydroxyphenylretinamide>nimesulide>9-cis-retinoic acid>polyphenon E. Diphenylhydramine, D-mannitol, and nordihydroguaiaretic acid were inactive. The results of these initial studies suggest that ultraviolet-B-treated radial growth-phase-like melanoma cells are the most responsive to chemopreventive agents, and may be the cell line of choice for screening melanoma prevention agents. |
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Authors:
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Eugene Elmore; Aarti Jain; Shazia Siddiqui; Nilou Tohidian; Frank L Meyskens; Vernon E Steele; John L Redpath |
Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural |
Journal Detail:
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Title: Melanoma research Volume: 17 ISSN: 0960-8931 ISO Abbreviation: Melanoma Res. Publication Date: 2007 Feb |
Date Detail:
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Created Date: 2007-01-19 Completed Date: 2007-04-03 Revised Date: 2007-12-03 |
Medline Journal Info:
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Nlm Unique ID: 9109623 Medline TA: Melanoma Res Country: England |
Other Details:
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Languages: eng Pagination: 42-50 Citation Subset: IM |
Affiliation:
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Department of Radiation Oncology, University of California, Irvine, California 92697-2695, USA. eelmore@uci.edu |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Annexin A5
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analysis Cadherins / analysis Cell Line Cell Line, Tumor Foreskin / cytology Humans Infant, Newborn Male Mass Screening / methods Melanocytes / cytology*, pathology Melanoma / epidemiology, prevention & control* Reproducibility of Results Skin Neoplasms / epidemiology, prevention & control* Ultraviolet Rays |
| Grant Support | |
ID/Acronym/Agency:
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CA62203/CA/NCI NIH HHS; N01-CN-15030/CN/NCI NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Annexin A5; 0/Cadherins |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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