Document Detail


Development, characterisation, and across-taxa utility of oil palm (Elaeis guineensis Jacq.) microsatellite markers.
MedLine Citation:
PMID:  11444700     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The results of the development of oil palm (Elaeis guineensis Jacq.) microsatellite markers are given step by step, from the screening of libraries enriched in (GA)n, (GT)n, and (CCG)n simple-sequence repeats (SSRs) to the final characterisation of 21 SSR loci. Also published are primer sequences, estimates of allele size range, and expected heterozygosity in E. guineensis and in the closely related species E. oleifera, in which an optimal utility of the SSR markers was observed. Multivariate data analyses showed the ability of SSR markers to efficiently reveal the genetic-diversity structure of the genus Elaeis in accordance with known geographical origins and with measured genetic relationships based on previous molecular studies. High levels of allelic variability indicated that E. guineensis SSRs will be a powerful tool for genetic studies of the genus Elaeis, including variety identification and intra- or inter-specific genetic mapping. PCR amplification tests on a subset of 16 other palm species and allele-sequence data showed that E. guineensis SSRs are putative transferable markers across palm taxa. In addition, phenetic information based on SSR flanking region sequences makes E. guineensis SSR markers a potentially useful molecular resource for any researcher studying the phylogeny of palm taxa.
Authors:
N Billotte; A M Risterucci; E Barcelos; J L Noyer; P Amblard; F C Baurens
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Genome / National Research Council Canada = Génome / Conseil national de recherches Canada     Volume:  44     ISSN:  0831-2796     ISO Abbreviation:  Genome     Publication Date:  2001 Jun 
Date Detail:
Created Date:  2001-07-10     Completed Date:  2001-12-07     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  8704544     Medline TA:  Genome     Country:  Canada    
Other Details:
Languages:  eng     Pagination:  413-25     Citation Subset:  IM    
Affiliation:
Centre de Cooperation Internationale en Recherche Agronomique pour le Développement, Montpellier, France. billotte@cirad.fr
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MeSH Terms
Descriptor/Qualifier:
Africa
Angiosperms / classification*,  genetics*
Asia
Base Sequence
Cloning, Molecular
DNA Primers
DNA, Plant / genetics
Databases, Nucleic Acid
Evolution, Molecular*
Genetic Variation
Genomic Library
Microsatellite Repeats / genetics*
Molecular Sequence Data
Phylogeny*
Polymerase Chain Reaction
Polymorphism, Genetic
Sequence Alignment
Sequence Homology, Nucleic Acid
South America
Chemical
Reg. No./Substance:
0/DNA Primers; 0/DNA, Plant

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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