| Development and characterisation of acellular allogeneic arterial matrices. | |
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MedLine Citation:
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PMID: 21919791 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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Surgeons have used cryopreserved vascular allografts successfully for many years to treat arterial occlusive disease and to repair arterial aneurysms. Vascular allografts demonstrate high patency rates but contain viable cells, which may evoke a rejection response following implantation. Removing the cells could prevent such a response and negate the need for cryopreservation and ultra low temperature storage. The objectives of the study were to characterise human common femoral arteries and develop a decellularisation protocol with a view to the generation of biocompatible and biomechanically functional vascular grafts for use in vascular bypass and arteriovenous access. The arteries were decellularised by subjecting the tissue to a freeze-thaw, incubation in hypotonic tris buffer, 0.1 % (w/v) sodium dodecyl sulphate in hypotonic buffer plus protease inhibitors, nucleases and hypertonic buffer followed by disinfection using 0.1 % (v/v) peracetic acid and final washing in phosphate buffered saline. Histological analysis demonstrated a lack of cells following decellularisation and confirmed the integrity of the tissue histioarchitecture and retention of major structural proteins. There was a >95 % reduction in DNA levels. The acellular tissues and extracts were not cytotoxic to either mouse 3T3 or baby hamster kidney cells. Biomechanical properties were determined by burst pressure, compliance, and tensile tests which confirmed the retention of biomechanical properties following decellularisation. In conclusion the study has developed a suitable protocol for the removal of cells from human common femoral arteries without adversely affecting the biochemical or biomechanical properties. These properties indicate the potential use for acellular human common femoral arteries for vascular bypass or arteriovenous access. |
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Authors:
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Stacy-Paul P Wilshaw; Paul Rooney; Helen Berry; John N Kearney; Shervanthi Homer-Vanniasinkam; John Fisher; Eileen Ingham |
Publication Detail:
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Type: JOURNAL ARTICLE Date: 2011-9-15 |
Journal Detail:
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Title: Tissue engineering. Part A Volume: - ISSN: 1937-335X ISO Abbreviation: - Publication Date: 2011 Sep |
Date Detail:
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Created Date: 2011-9-16 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 101466659 Medline TA: Tissue Eng Part A Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Affiliation:
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University of Leeds, Institute of Medical and Biological Engineering, Faculty of Biological Sciences, Mount preston Street, Leeds, West Yorkshire, United Kingdom, LS2 9JT, 0113 3435651, 0113 3435651; s.wilshaw@leeds.ac.uk. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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