| Development of a cell wash buffer that minimizes nucleic acid loss from Clostridium perfringens 10543 A. | |
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MedLine Citation:
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PMID: 2411369 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Autolytic activity and nucleic loss from Clostridium perfringens 10543 A was demonstrated during successive cell washes in hypotonic TES buffer. Autolysis increased nearly sixfold and nucleic acid loss nearly twofold when 10 mM EDTA was added to 0.3 M Tris-sucrose buffer. Attempts to minimize both autolysis and nucleic acid loss from C. perfringens during routine washing steps were unsuccessful when the effects of sucrose concentration, pH, CaCl2 addition, or wash temperature were examined independently. However, autolytic activity was eliminated and nucleic acid loss reduced to less than 5% when C. perfringens cells were washed at 4 or 25 degrees C in 1.0 M sucrose, 50 mM Tris--HCl, and 25 mM CaCl2 at pH 5.7. |
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Authors:
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H P Blaschek; M A Klacik |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Canadian journal of microbiology Volume: 31 ISSN: 0008-4166 ISO Abbreviation: Can. J. Microbiol. Publication Date: 1985 Jun |
Date Detail:
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Created Date: 1985-09-30 Completed Date: 1985-09-30 Revised Date: 2007-11-14 |
Medline Journal Info:
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Nlm Unique ID: 0372707 Medline TA: Can J Microbiol Country: CANADA |
Other Details:
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Languages: eng Pagination: 575-8 Citation Subset: IM |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Bacteriological Techniques* Bacteriolysis Calcium Chloride / pharmacology Clostridium perfringens / physiology* DNA, Bacterial / metabolism* Edetic Acid / pharmacology Hydrogen-Ion Concentration Hypotonic Solutions Nucleic Acids / metabolism Osmotic Pressure RNA, Bacterial / metabolism* Sucrose / pharmacology Tromethamine / pharmacology |
| Grant Support | |
ID/Acronym/Agency:
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1-5-24295//PHS HHS |
| Chemical | |
Reg. No./Substance:
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0/DNA, Bacterial; 0/Hypotonic Solutions; 0/Nucleic Acids; 0/RNA, Bacterial; 10043-52-4/Calcium Chloride; 57-50-1/Sucrose; 60-00-4/Edetic Acid; 77-86-1/Tromethamine |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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