| Development of capillary gas chromatographic-mass spectrometric methodology for the simultaneous determination of ibuprofen and [ar-2H4]ibuprofen in serum: demonstration of kinetic equivalence in the beagle. | |
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MedLine Citation:
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PMID: 3745396 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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A capillary gas chromatographic-mass spectrometric method for the determination of ibuprofen and tetra-deuterated ibuprofen in serum is described. Ibuprofen, [ar-2H4]ibuprofen and the internal standard, [ar-2H4,3,3,3-2H3]ibuprofen, are extracted (after acidification) from serum onto a cross-linked styrene divinyl benzene resin by an automated sample processor. After elution and evaporation of the organic phase, samples are reconstituted with solvent and analyzed without derivatization by capillary gas chromatography-mass spectrometry. This methodology was used to evaluate possible kinetic isotope effects after the coadministration of an equimolar mixture of ibuprofen and the deuterium-labeled covariant in the beagle. No significant differences in absorption or elimination were observed. |
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Authors:
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D L Theis; G W Halstead; K A Halm |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Journal of chromatography Volume: 380 ISSN: 0021-9673 ISO Abbreviation: J. Chromatogr. Publication Date: 1986 Jul |
Date Detail:
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Created Date: 1986-10-02 Completed Date: 1986-10-02 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0427043 Medline TA: J Chromatogr Country: NETHERLANDS |
Other Details:
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Languages: eng Pagination: 77-87 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Chromatography, Gas Dogs Gas Chromatography-Mass Spectrometry Ibuprofen / blood* Kinetics Therapeutic Equivalency |
| Chemical | |
Reg. No./Substance:
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15687-27-1/Ibuprofen |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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