Document Detail


Development of breed identification markers derived from AFLP in beef cattle.
MedLine Citation:
PMID:  22061324     Owner:  NLM     Status:  In-Data-Review    
Abstract/OtherAbstract:
In the meat industry, correct breed information in food labeling is required to assure meat quality. Genetic markers provide corroborating evidence to identify breed. This paper describes the development of DNA markers to discriminate between Japanese Black and F1 (Japanese Black×Holstein) breeds. Amplified fragment length polymorphism method was employed to detect candidate markers absent in Japanese Black but present in Holstein. The 500 primer combinations yielded six selected markers that were converted into single nucleotide polymorphisms markers for high-throughput genotyping. The allele frequencies in both breeds were investigated for discrimination ability using PCR-RFLP. The probability of identifying F1 was 0.882 and probability of misjudgment was 0.0198. The markers could be useful for discriminating between Japanese Black and F1 and would contribute to the elimination of falsified breed labeling of meat.
Authors:
S Sasazaki; K Itoh; S Arimitsu; T Imada; A Takasuga; H Nagaishi; S Takano; H Mannen; S Tsuji
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Meat science     Volume:  67     ISSN:  0309-1740     ISO Abbreviation:  Meat Sci.     Publication Date:  2004 Jun 
Date Detail:
Created Date:  2011-11-08     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101160862     Medline TA:  Meat Sci     Country:  England    
Other Details:
Languages:  eng     Pagination:  275-80     Citation Subset:  -    
Affiliation:
Laboratory of Animal Breeding and Genetics, Graduate School of Science and Technology, Kobe University, Kobe 657-8501, Japan.
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