Document Detail


Development of biosensors containing laccase and imidazolium bis(trifluoromethylsulfonyl)imide ionic liquid for the determination of rutin.
MedLine Citation:
PMID:  19345764     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Biosensors based on hydrophobic ionic liquids (ILs) derived from the bis(trifluoromethylsulfonyl)imide [(CF(3)SO(2))(2)N(-) = Tf(2)N(-)] anion associated with three different imidazolium cations: 1-butyl-3-methylimidazolium (BMI x Tf(2)N), 1-decyl-3-methylimidazolium (DMI x Tf(2)N) and 1-tetradecyl-3-methylimidazolium (TDMI x Tf(2)N), along with laccase from Aspergillus oryzae, were constructed and optimized for determination of rutin. The laccase catalyzes the oxidation of rutin to the corresponding o-quinone, which is electrochemically reduced back to rutin. The best performance was obtained with 50:20:15:15% (w/w/w/w) as the graphite powder:laccase:Nujol:ILs composition in 0.1 mol L(-1) acetate buffer solution (pH 5.0). The parameters for the square-wave voltammetry experiments and scanning electron microscopy images of the biosensors were studied. Under the selected conditions, the cathodic peak current increased linearly in the rutin concentration ranges of 4.77x10(-6) to 4.62x10(-5) mol L(-1), 5.84x10(-6) to 5.36x10(-5) mol L(-1) and 5.84x10(-6) to 5.36x10(-5) mol L(-1) using the (I) BMI x Tf(2)N-laccase, (II) DMI x Tf(2)N-laccase and (III) TDMI x Tf(2)N-laccase, respectively. The rutin contents of commercial samples of pharmaceuticals were successfully determined by the biosensors and the results compared well with those obtained using the official method. The studies on rutin recovery from these samples gave values of 96.9-104.6%.
Authors:
Ana Cristina Franzoi; Pedro Migowski; Jairton Dupont; Iolanda Cruz Vieira
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2009-03-18
Journal Detail:
Title:  Analytica chimica acta     Volume:  639     ISSN:  1873-4324     ISO Abbreviation:  Anal. Chim. Acta     Publication Date:  2009 Apr 
Date Detail:
Created Date:  2009-04-06     Completed Date:  2009-07-02     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0370534     Medline TA:  Anal Chim Acta     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  90-5     Citation Subset:  IM    
Affiliation:
Departamento de Química, Laboratório de Biossensores, Universidade Federal de Santa Catarina, 88040-970 Florianópolis, SC, Brazil.
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MeSH Terms
Descriptor/Qualifier:
Aspergillus oryzae / enzymology
Biosensing Techniques / methods*
Electrochemistry
Hydrogen-Ion Concentration
Hydrophobicity
Imidazoles / chemistry*
Imides / chemistry*
Ionic Liquids / chemistry*
Laccase / metabolism*
Microscopy, Electron, Scanning
Pharmaceutical Preparations / chemistry
Reproducibility of Results
Rutin / analysis*
Chemical
Reg. No./Substance:
0/Imidazoles; 0/Imides; 0/Ionic Liquids; 0/Pharmaceutical Preparations; 153-18-4/Rutin; EC 1.10.3.2/Laccase

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