| Development of a biosensor for caffeine. | |
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MedLine Citation:
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PMID: 17386510 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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We have utilized a microbe, which can degrade caffeine to develop an Amperometric biosensor for determination of caffeine in solutions. Whole cells of Pseudomonas alcaligenes MTCC 5264 having the capability to degrade caffeine were immobilized on a cellophane membrane with a molecular weight cut off (MWCO) of 3000-6000 by covalent crosslinking method using glutaraledhyde as the bifunctional crosslinking agent and gelatin as the protein based stabilizing agent (PBSA). The biosensor system was able to detect caffeine in solution over a concentration range of 0.1 to 1 mg mL(-1). With read-times as short as 3 min, this caffeine biosensor acts as a rapid analysis system for caffeine in solutions. Interestingly, successful isolation and immobilization of caffeine degrading bacteria for the analysis of caffeine described here was enabled by a novel selection strategy that incorporated isolation of caffeine degrading bacteria capable of utilizing caffeine as the sole source of carbon and nitrogen from soils and induction of caffeine degrading capacity in bacteria for the development of the biosensor. This biosensor is highly specific for caffeine and response to interfering compounds such as theophylline, theobromine, paraxanthine, other methyl xanthines and sugars was found to be negligible. Although a few biosensing methods for caffeine are reported, they have limitations in application for commercial samples. The development and application of new caffeine detection methods remains an active area of investigation, particularly in food and clinical chemistry. The optimum pH and temperature of measurement were 6.8 and 30+/-2 degrees C, respectively. Interference in analysis of caffeine due to different substrates was observed but was not considerable. Caffeine content of commercial samples of instant tea and coffee was analyzed by the biosensor and the results compared well with HPLC analysis. |
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Authors:
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V R Sarath Babu; S Patra; N G Karanth; M A Kumar; M S Thakur |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2006-09-27 |
Journal Detail:
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Title: Analytica chimica acta Volume: 582 ISSN: 1873-4324 ISO Abbreviation: Anal. Chim. Acta Publication Date: 2007 Jan |
Date Detail:
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Created Date: 2007-03-27 Completed Date: 2007-05-31 Revised Date: 2007-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0370534 Medline TA: Anal Chim Acta Country: Netherlands |
Other Details:
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Languages: eng Pagination: 329-34 Citation Subset: IM |
Affiliation:
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Fermentation Technology and Bioengineering Department, Central Food Technological Research Institute, Mysore 570020, India. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Biosensing Techniques* Caffeine / analysis* Calibration Chromatography, High Pressure Liquid Enzymes, Immobilized / chemistry Hydrogen-Ion Concentration Pseudomonas alcaligenes / chemistry* Sensitivity and Specificity Temperature Xanthine Oxidase / chemistry |
| Chemical | |
Reg. No./Substance:
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0/Enzymes, Immobilized; 58-08-2/Caffeine; EC 1.17.3.2/Xanthine Oxidase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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