Document Detail

Development of a biomolecular assay for the identification of Listeria at species level.
MedLine Citation:
PMID:  20446860     Owner:  NLM     Status:  MEDLINE    
In this article, we developed a biomolecular assay specifically designed for the identification of Listeria at species level. The proposed test was based on (i) a duplex PCR targeting a specific fragment for L. monocytogenes (plcA gene) and a common fragment for all the Listeria (16S rRNA gene) and (ii) a minisequencing of the common fragment to detect diagnostic sites for the differentiation of the other five species: L. innocua, L. welshimeri, L. seeligeri, L. ivanovii, and L. grayi. The specificity of the assay was first tested on a total of 25 certified strains representing 6 Listeria species and 14 non-Listeria strains as negative control and validated on 124 wild isolates obtained from food samples. The proposed assay provides an appropriate tool for rapid identification of Listeria at species level and it should be of great benefit to the food industry as well as to regulatory or public health laboratories engaged in establishing the safety of food products and the management of listeriosis.
Alessandra Dalmasso; Kalliopi Rantsiou; Luca Cocolin; Maria Teresa Bottero
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Foodborne pathogens and disease     Volume:  7     ISSN:  1556-7125     ISO Abbreviation:  Foodborne Pathog. Dis.     Publication Date:  2010 May 
Date Detail:
Created Date:  2010-05-07     Completed Date:  2010-08-20     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101120121     Medline TA:  Foodborne Pathog Dis     Country:  United States    
Other Details:
Languages:  eng     Pagination:  565-71     Citation Subset:  IM    
Department of Animal Pathology, Faculty of Veterinary Medicine, University of Turin, Grugliasco, Italy.
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MeSH Terms
Base Sequence
DNA, Bacterial / isolation & purification
Databases, Nucleic Acid
Food Industry / methods
Food Microbiology
Listeria / enzymology,  genetics*,  isolation & purification*
Listeria Infections / microbiology,  prevention & control
Listeria monocytogenes / enzymology,  genetics,  isolation & purification
Molecular Sequence Data
Polymerase Chain Reaction / methods*
RNA, Ribosomal, 16S / chemistry,  genetics*
Sequence Alignment
Sequence Analysis, RNA
Species Specificity
Type C Phospholipases / genetics*
Reg. No./Substance:
0/DNA, Bacterial; 0/RNA, Ribosomal, 16S; EC 3.1.4.-/Type C Phospholipases

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