| Development and application of a novel acridinium ester for use as a chemiluminescent emitter in nucleic acid hybridisation assays using chemiluminescence quenching. | |
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MedLine Citation:
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PMID: 19109686 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Chemiluminescent acridinium esters (AEs) permit the development of high sensitivity ligand binding assays due to a combination of high intensity light emission and very low backgrounds. Here these advantages are exploited for use in homogeneous nucleic acid hybridisation assays using quenched chemiluminescence. AE chemiluminescence is conventionally initiated at highly alkaline pH. Novel "active" AEs were designed that permit initiation under conditions compatible with maintenance of nucleic acid hybrids (i.e. pH less than 9). Methyl red was found to be a dark quencher species capable of functioning at this pH. Practical application of the chemiluminescence quenching assay system has been demonstrated using two model nucleic acid hybridisation assays based on intra- and intermolecular emitter/quencher pairs. |
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Authors:
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Richard C Brown; Zhaoqiang Li; Andrew J Rutter; Xiaojing Mu; Owen H Weeks; Keith Smith; Ian Weeks |
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Publication Detail:
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Type: Journal Article Date: 2008-11-24 |
Journal Detail:
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Title: Organic & biomolecular chemistry Volume: 7 ISSN: 1477-0539 ISO Abbreviation: Org. Biomol. Chem. Publication Date: 2009 Jan |
Date Detail:
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Created Date: 2008-12-25 Completed Date: 2009-02-13 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 101154995 Medline TA: Org Biomol Chem Country: England |
Other Details:
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Languages: eng Pagination: 386-94 Citation Subset: IM |
Affiliation:
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Molecular Light Technology Research Limited, (Subsidiary of Gen-Probe Incorporated), 10210 Genetic Center Drive, San Diego, CA 92121, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Acridines
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chemistry* Azo Compounds / chemistry Chemiluminescent Measurements / methods* Esters / chemistry Hydrogen-Ion Concentration Luminescence Nucleic Acid Hybridization / methods* |
| Chemical | |
Reg. No./Substance:
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0/Acridines; 0/Azo Compounds; 0/Esters; 493-52-7/methyl red |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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