Document Detail


Development and application of a novel UV method for the analysis of ascorbic acid.
MedLine Citation:
PMID:  15620539     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A UV method for the analysis of ascorbic acid with methanol as solvent to prepare a sample has been developed and applied. The effect of copper(II) concentrations on the oxidation of ascorbic acid in aqueous solution has been studied in detail, and the regularities of ascorbic acid oxidation in methanol, USP phosphate buffer (pH 2.50) and de-ionized water have been found. Upon experiments ascorbic acid has been found to dissolve in methanol, and its solubility in it has been measured to be 81.0mg/ml at room temperature (22 degrees C). The ascorbic acid bulk material from a manufacturer has been assayed to be 89.34% with this method, in good agreement with the assay value (89.58%) from the titration method. The ascorbic acid granule and tablet content uniformity also has been tested using this method. This method is simple, rapid, accurate and reliable, and can be adopted for the routine determination of ascorbic acid in its granule and tablet formulations.
Authors:
Wenming Zeng; Frank Martinuzzi; Alexander MacGregor
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of pharmaceutical and biomedical analysis     Volume:  36     ISSN:  0731-7085     ISO Abbreviation:  J Pharm Biomed Anal     Publication Date:  2005 Jan 
Date Detail:
Created Date:  2004-12-28     Completed Date:  2005-06-03     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8309336     Medline TA:  J Pharm Biomed Anal     Country:  England    
Other Details:
Languages:  eng     Pagination:  1107-11     Citation Subset:  IM    
Affiliation:
Research and Development, Toronto Institute of Pharmaceutical Technology, 55 Town Center Court-Suite 200, Toronto, Ont., Canada M1P 4X4. wenming@tipt.com
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MeSH Terms
Descriptor/Qualifier:
Ascorbic Acid / analysis*,  metabolism
Chemistry, Pharmaceutical / methods*
Copper / metabolism
Oxidation-Reduction
Spectrophotometry, Ultraviolet / methods
Chemical
Reg. No./Substance:
50-81-7/Ascorbic Acid; 7440-50-8/Copper

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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